Shi Yu, Liu Junjun, Liu Yingying, Quan Hong, Li Bo, Lu Haili, Ding Hanzhi, Yu Zuoren, Han Jing
Department of Breast Cancer, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
Department of Physiology and Pathophysiology, Health Science Center, Peking University, Beijing 100191, China.
Heliyon. 2023 Jul 26;9(8):e18704. doi: 10.1016/j.heliyon.2023.e18704. eCollection 2023 Aug.
Monitoring abnormal viscosity in biological systems is important for basic research and clinical applications. Fluorescence imaging technology is adaptable for the visualization of tumor tissues due to its comprehensive features. However, fluorescence detection of intracellular viscosity in clinical samples remains challenging. We developed a promising near-infrared fluorescent probe, M556, for viscosity measurement. M556, which targets mitochondria, was successfully applied to monitor the mitochondrial viscosity in living cells. Furthermore, M556 was demonstrated to effectively discriminate tumors from normal tissues in a mouse tumor model and in clinical specimens from breast cancer patients, thus indicating the potential perioperative use of this probe by clinicians to assist with biopsy procedures.
监测生物系统中的异常粘度对于基础研究和临床应用都很重要。荧光成像技术因其综合特性而适用于肿瘤组织的可视化。然而，临床样本中细胞内粘度的荧光检测仍然具有挑战性。我们开发了一种有前景的近红外荧光探针M556用于粘度测量。靶向线粒体的M556成功应用于监测活细胞中的线粒体粘度。此外，在小鼠肿瘤模型和乳腺癌患者的临床标本中，M556被证明能有效区分肿瘤与正常组织，从而表明临床医生在围手术期使用该探针辅助活检程序的潜力。
