喀麦隆 2013-2016 年流行麻疹病毒株的分子特征。
Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics.
机构信息
WHO National Measles Reference Laboratory, Department of Virology, Centre Pasteur of Cameroon (CPC), Yaoundé, Centre, Cameroon.
Faculty of Science, University of Yaoundé 1, Yaoundé, Centre, Cameroon.
出版信息
PLoS One. 2019 Sep 25;14(9):e0222428. doi: 10.1371/journal.pone.0222428. eCollection 2019.
The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in certain regions, hence the need for an update. The objective of this study was to have recent data on MeV genotypes circulating in Cameroon. Ninety throat swabs collected during recent measles outbreaks were analyzed by MeV genotyping RT-PCR using the nucleoprotein gene N. The resulting sequences were analyzed on the basis of 450 nucleotides with MEGA 7 software. Overall genome analysis was performed on 40/90 sequences. The strains were from all ten regions and all belonged to cluster 1 of genotype B3. The genotype B3 has been circulating in Cameroon for long periods of time; efforts must be made in immunization for its elimination.
关于喀麦隆麻疹病毒 (MeV) 株的第一代基因分型数据可追溯到 1994 年，而其他研究则是在 2001 年和 2011 年建立 MeV 病毒学监测后进行的。然而，在喀麦隆流行的 MeV 株的遗传数据仍然分散且集中在某些地区，因此需要更新。本研究的目的是获得喀麦隆最近流行的 MeV 基因型数据。使用麻疹病毒基因分型 RT-PCR 用核蛋白基因 N 分析了最近麻疹暴发期间采集的 90 个咽喉拭子。使用 MEGA 7 软件对得到的序列基于 450 个核苷酸进行分析。对 40/90 个序列进行了全基因组分析。这些菌株来自十个地区的所有地区，都属于基因型 B3 的簇 1。B3 基因型已经在喀麦隆流行了很长时间；必须在免疫方面做出努力以消除它。
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