Laboratory of Clinical Virology and WHO Regional Reference Laboratory for Poliomyelitis and Measles in Eastern Mediterranean Region, Institut Pasteur, Tunis, Tunisia.
Virus Res. 2010 Nov;153(2):258-64. doi: 10.1016/j.virusres.2010.08.011. Epub 2010 Aug 20.
Genetic characterization was conducted on 18 wild-type measles viruses, detected in Tunisia and Libya from 2002 to 2009. Sequence analysis of the 456 nucleotides in the carboxy terminus of the nucleoprotein (N) gene and the entire hemagglutinin (H) gene indicated that all isolates were in genotype B3. All of the viruses from 2002 to 2007 and some of the isolates from 2009 belonged to subtype B3.1. In contrast, 7 of the viruses isolated during 2008 and 2009 were quite divergent from all B3 isolates. The nucleotide sequences of the N gene of these 7 isolates differed from the sequences of the Ibadan and New York reference strain by an average of 3.1 and 4.4%, respectively. The H gene sequences differed by 1.1 and 2.6% with the same reference strains. This is the first report describing the genetic characteristics of measles viruses from clade B isolated in North Africa; the results suggest that these viruses represent a new subtype of genotype B3.
对 2002 年至 2009 年在突尼斯和利比亚检测到的 18 株野生型麻疹病毒进行了遗传特征分析。对核蛋白（N）基因羧基末端的 456 个核苷酸和整个血凝素（H）基因的序列分析表明，所有分离株均属于基因型 B3。2002 年至 2007 年的所有病毒和 2009 年的部分分离株属于 B3.1 亚型。相比之下，2008 年和 2009 年分离的 7 株病毒与所有 B3 分离株存在较大差异。这 7 株分离株的 N 基因核苷酸序列与伊巴丹和纽约参考株的序列平均分别相差 3.1%和 4.4%。H 基因序列与相同的参考株相差 1.1%和 2.6%。这是首次描述北非分离的 B 谱系麻疹病毒的遗传特征；结果表明这些病毒代表了基因型 B3 的一个新亚型。
