Buxbaum P, Horvat G, Gamper C, Krisch K
Department of Pathology, University of Vienna, Medical School, Austria.
Cancer Detect Prev Suppl. 1987;1:263-8.
The monoclonal islet cell antibody HISL-19 generated after immunization of BALB/c mice with human pancreatic islet cell preparations, demonstrated specific immunoreactivity for neuroendocrine (Merkel) cells of the skin as shown by successive and simultaneous localization of neuron-specific enolase and the antigen detected by mab HISL-19 in the same cells of the bovine epidermis. Following these observations, we tested nine neuroendocrine carcinomas of the skin that were believed to be of Merkel cell origin for their immunoreactivity with mab HISL-19 using an indirect immunoperoxidase technique on formalin-fixed and paraplast-embedded tissues. In contrast to malignant lymphomas, poorly differentiated squamous cell carcinomas, and malignant melanomas, all nine neuroendocrine carcinomas reacted strongly with mab HISL-19, indicating its potential as a useful immunohistochemical probe for the distinction of neuroendocrine carcinomas of the skin from other cutaneous neoplasms with similar histological appearance.
用人类胰岛细胞制剂免疫BALB/c小鼠后产生的单克隆胰岛细胞抗体HISL-19，对皮肤的神经内分泌（默克尔）细胞表现出特异性免疫反应性，如在牛表皮的相同细胞中神经元特异性烯醇化酶和mab HISL-19检测到的抗原的连续和同时定位所示。基于这些观察结果，我们使用间接免疫过氧化物酶技术，对福尔马林固定和石蜡包埋组织中9例被认为起源于默克尔细胞的皮肤神经内分泌癌进行了mab HISL-19免疫反应性检测。与恶性淋巴瘤、低分化鳞状细胞癌和恶性黑色素瘤不同，所有9例神经内分泌癌均与mab HISL-19发生强烈反应，表明其有潜力作为一种有用的免疫组织化学探针，用于区分皮肤神经内分泌癌与其他具有相似组织学外观的皮肤肿瘤。
