Bordi C, Krisch K, Horvat G, Srikanta S
Institute of Pathological Anatomy, University of Parma, Italy.
Am J Pathol. 1988 Aug;132(2):249-57.
A series of 51 islet cell tumors removed from 28 patients was investigated immunohistochemically with the monoclonal antibody HISL-19. The antibody was produced after immunization of BALB/c mice with human islet cells and was found to react with a wide range of neuroendocrine and neural cells. All tumors presented positive immunoreaction showing various combinations of 2 basic patterns. The first pattern reflected the immunostaining of the secretory granules of the tumor cells. This "granular" staining was predominantly associated with benign neoplasms and with the tumoral production of glucagon and pancreatic polypeptide (PP), while it was absent or inconsistent in most insulin-secreting tumors. The second pattern consisted of focal immunoreactive aggregates located in a peri- (and, in polarized cells, supra-) nuclear position. This "cluster-type" staining showed a good morphologic and topographic correspondence with the Golgi apparatus of the cells of the same tumors, as shown by electron microscopy. The latter pattern was well represented in all types of islet cell tumors except those producing PP. Moreover, it was more apparent in less differentiated tumors in which the granular pattern was often absent or inconsistent. Cluster-type (but not granular) immunoreactivity was frequently found in some nonendocrine, nontumoral pancreatic structures, particularly in the epithelium of small ducts. However, the immunoreactive aggregates of nonendocrine cells were distinctly less prominent than those of endocrine cells. On the basis of a comparison with other immunohistochemical markers for neuroendocrine cells, it is concluded that the HISL-19 monoclonal antibody presents specific staining characteristics useful for the cytologic analysis of islet cell tumors.
用单克隆抗体HISL-19对从28例患者身上切除的51个胰岛细胞瘤进行了免疫组织化学研究。该抗体是用人类胰岛细胞免疫BALB/c小鼠后产生的，发现它能与多种神经内分泌和神经细胞发生反应。所有肿瘤均呈现阳性免疫反应，显示出两种基本模式的各种组合。第一种模式反映了肿瘤细胞分泌颗粒的免疫染色。这种“颗粒状”染色主要与良性肿瘤以及肿瘤性胰高血糖素和胰多肽（PP）的产生有关，而在大多数胰岛素分泌性肿瘤中则不存在或不一致。第二种模式由位于核周（以及在极化细胞中位于核上）位置的局灶性免疫反应性聚集物组成。如电子显微镜所示，这种 “簇状” 染色在形态和拓扑结构上与同一肿瘤细胞的高尔基体具有良好的对应关系。除了产生PP的肿瘤外，后一种模式在所有类型的胰岛细胞瘤中都有很好的体现。此外，在分化程度较低的肿瘤中更明显，在这些肿瘤中颗粒状模式往往不存在或不一致。在一些非内分泌、非肿瘤性胰腺结构中，特别是在小导管上皮中，经常发现簇状（而非颗粒状）免疫反应性。然而，非内分泌细胞的免疫反应性聚集物明显不如内分泌细胞的突出。通过与其他神经内分泌细胞免疫组织化学标志物的比较得出结论，HISL-19单克隆抗体具有特定的染色特征，可用于胰岛细胞瘤的细胞学分析。
