Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal; Laboratory of Separation and Reaction Engineering, Laboratory of Catalysis and Materials (LSRE-LCM), Bragança Polytechnic Institute, 5301-857 Bragança, Portugal; Grupo de Investigación en Polifenoles (GIP-USAL), Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno s/n, 37007 Salamanca, Spain.
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal; Department of Biochemistry, State University of Maringá, Paraná, Brazil; Graduate Program in Food Science, State University of Maringá, Paraná, Brazil.
Food Res Int. 2019 Nov;125:108541. doi: 10.1016/j.foodres.2019.108541. Epub 2019 Jul 10.
Response surface methodology was applied to optimize the recovery of ergosterol from an agroindustrial residue of Agaricus blazei Murrill, by comparing conventional (heat-assisted extraction, HAE) and more sustainable non-conventional approaches (ultrasound and microwave-assisted extractions, UAE and MAE, respectively). A circumscribed central composite design was employed to optimize and describe the interactive effects among variables of the HAE, MAE (time and temperature) and UAE systems (time and ultrasound power), and their performances compared with the standard soxhlet extraction system. The optimum conditions were predicted as 150 min, 81.6 °C for HAE, 30 min, 400 W for UAE, and 25 min, 134.6 °C, for the MAE system. The UAE system was identified as the most appropriate technique to obtain extracts with the highest amount of ergosterol, followed by MAE and HAE. Overall, these results demonstrate the potential of using the fruiting bodies of A. blazei as a suitable source to obtain ergosterol-rich extracts, which can be used as an ingredient for various bio-based industrial applications.
响应面法被应用于优化从 Agaricus blazei Murrill 的农业工业残余物中回收麦角固醇，通过比较传统（热辅助提取，HAE）和更可持续的非传统方法（超声和微波辅助提取，UAE 和 MAE）。采用限定中心复合设计来优化和描述 HAE、MAE（时间和温度）和 UAE 系统（时间和超声功率）之间变量的相互作用，并将其性能与标准索氏提取系统进行比较。最佳条件预测为 HAE 为 150 分钟，81.6°C；UAE 为 30 分钟，400W；MAE 为 25 分钟，134.6°C。UAE 系统被确定为获得麦角固醇含量最高的提取物的最合适技术，其次是 MAE 和 HAE。总的来说，这些结果表明，使用 A. blazei 的子实体作为获得富含麦角固醇的提取物的合适来源具有潜力，可将其用作各种基于生物的工业应用的成分。
