（邦格。）施图德。源自叶片的提取物作为有效的抗登革热2型血清型药物。

(Bong.) Steud. leaves-derived extracts as potent anti-dengue serotype 2.

作者信息

Dos Santos Michelli, Teixeira Thaiz Rodrigues, Santos Felipe Rocha da Silva, Lima William Gustavo, Ferraz Ariane Coelho, Silva Nathália Lucca, Leite Flávio José, Siqueira João Máximo, Luyten Walter, de Castro Ana Hortência Fonseca, de Magalhães José Carlos, Ferreira Jaqueline Maria Siqueira

机构信息

Laboratório de Microbiologia Médica, Universidade Federal de São João del-Rei, Divinópolis, Brazil.

Laboratório de Química Orgânica do Ambiente Marinho. Departamento de Física e Química da Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto, Brazil.

出版信息

Nat Prod Res. 2021 Aug;35(16):2804-2809. doi: 10.1080/14786419.2019.1669030. Epub 2019 Sep 26.

DOI:10.1080/14786419.2019.1669030

PMID:31554433

Abstract

(DENV) is the most prevalent mosquito-borne viral pathogen and made the disease a major health concern worldwide. However, specific antiviral drugs against this arbovirose or vaccines are not yet available for treatment or prevention. Thus, here we aimed to study the antiviral activity of hydroethanolic extract, fraction ethyl acetate and subfractions of the leaves of (Fabaceae:Cercideae), a native plant of the Brazilian Cerrado, against DENV-2 by methylthiazolyldiphenyl-tetrazolium bromide (MTT) method in mammalian cells culture. As results, the hydroethanolic extract showed the most potent effect, with an inhibitory concentration (IC) of 3.2 μg mL and selectivity index (SI) of 27.6, approximately 16-times higher anti-DENV-2 activity than of the ribavirin (IC 52.8 μg mL). Our results showed in this study appointed that has a promising anti-dengue activity, which was associated mainly with the presence of flavonoids.

摘要

登革病毒（DENV）是最常见的蚊媒病毒病原体，使该疾病成为全球主要的健康问题。然而，目前尚无针对这种虫媒病毒病的特效抗病毒药物或疫苗用于治疗或预防。因此，我们旨在通过甲基噻唑基二苯基四氮唑溴盐（MTT）法，在哺乳动物细胞培养中研究巴西塞拉多本土植物（豆科：决明科）叶片的水乙醇提取物、乙酸乙酯馏分和亚馏分对登革病毒2型（DENV-2）的抗病毒活性。结果显示，水乙醇提取物效果最为显著，其抑制浓度（IC）为3.2μg/mL，选择性指数（SI）为27.6，抗DENV-2活性约为利巴韦林（IC 52.8μg/mL）的16倍。我们在本研究中的结果表明，该植物具有有前景的抗登革热活性，这主要与黄酮类化合物的存在有关。