Srisala Supanart, Pongsakul Nutkridta, Sahakijpicharn Thiantip, Hongeng Suradej, Chutipongtanate Somchai, Apiwattanakul Nopporn
Section for Research Center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 270 Rama VI Road, Bangkok, 10400, Thailand.
Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 270 Rama VI Road, Bangkok, 10400, Thailand.
BMC Res Notes. 2019 Sep 26;12(1):633. doi: 10.1186/s13104-019-4659-4.
Capillary blood has been increasingly used in point-of-care setting for clinical monitoring in immunology and infectious diseases. We explored whether percentages of lymphocyte subsets (T-cells; CD3+, helper T-cells; CD4+, cytotoxic T-cells; CD8+, B-cells; CD19+, NK cells; CD56+, gamma delta T-cells, and regulatory T-cells) with regard to total lymphocyte count from capillary and venous blood of healthy volunteers were in good agreement.
All percentages of lymphocyte subsets with regard to total lymphocyte count from capillary blood were significantly correlated with those from venous blood (r ≥ 0.9 for every cell type). However, Bland-Altman plots showed high agreement between capillary and venous samples only in those of CD3+, CD4+, and CD8+ cells (limit of agreement percentages from mean venous blood < 20%). However, the agreement of percentages of other lymphocyte subsets from venous and capillary blood was mediocre. We concluded that capillary blood could be used as an alternative for venous blood to determine percentages of CD3+, CD4+, and CD8+ cells with regard to total lymphocyte count.
在免疫和传染病的临床监测中，毛细血管血越来越多地用于即时检测。我们探讨了健康志愿者毛细血管血和静脉血中淋巴细胞亚群（T细胞；CD3 +、辅助性T细胞；CD4 +、细胞毒性T细胞；CD8 +、B细胞；CD19 +、自然杀伤细胞；CD56 +、γδT细胞和调节性T细胞）占总淋巴细胞计数的百分比是否具有良好的一致性。
毛细血管血中淋巴细胞亚群占总淋巴细胞计数的所有百分比与静脉血中的百分比均显著相关（每种细胞类型的r≥0.9）。然而，Bland - Altman图显示，仅在CD3 +、CD4 +和CD8 +细胞的毛细血管血和静脉血样本之间具有高度一致性（相对于平均静脉血的一致性百分比极限<20%）。然而，静脉血和毛细血管血中其他淋巴细胞亚群百分比的一致性一般。我们得出结论，毛细血管血可作为静脉血的替代物，用于确定CD3 +、CD4 +和CD8 +细胞占总淋巴细胞计数的百分比。
