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冷缺血预处理对猪肾移植在常温机器灌流后加重缺血再灌注损伤。

Cold Preflush of Porcine Kidney Grafts Prior to Normothermic Machine Perfusion Aggravates Ischemia Reperfusion Injury.

机构信息

Department of Anesthesiology, Medical Faculty, RWTH Aachen University, Aachen, Germany.

Department of Intensive Care and Intermediate Care, Medical Faculty, RWTH Aachen University, Aachen, Germany.

出版信息

Sci Rep. 2019 Sep 25;9(1):13897. doi: 10.1038/s41598-019-50101-7.

DOI:10.1038/s41598-019-50101-7
PMID:31554887
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6761287/
Abstract

Normothermic machine perfusion (NMP) of kidney grafts is a promising new preservation method to improve graft quality and clinical outcome. Routinely, kidneys are washed out of blood remnants and cooled using organ preservation solutions prior to NMP. Here we assessed the effect of cold preflush compared to direct NMP. After 30 min of warm ischemia, porcine kidneys were either preflushed with cold histidine-tryptophan-ketoglutarate solution (PFNMP group) prior to NMP or directly subjected to NMP (DNMP group) using a blood/buffer solution. NMP was performed at a perfusion pressure of 75 mmHg for 6 h. Functional parameters were assessed as well as histopathological and biochemical analyses. Renal function as expressed by creatinine clearance, fractional excretion of sodium and total output of urine was inferior in PFNMP. Urine protein and neutrophil gelatinase-associated lipocalin (NGAL) concentrations as markers for kidney damage were significantly higher in the PFNMP group. Additionally, increased osmotic nephropathy was found after PFNMP. This study demonstrated that cold preflush prior to NMP aggravates ischemia reperfusion injury in comparison to direct NMP of warm ischemia-damaged kidney grafts. With increasing use of NMP systems for kidneys and other organs, further research into graft flushing during retrieval is warranted.

摘要

常温机械灌注（NMP）是一种有前途的新的保存方法，可以改善移植物的质量和临床结果。通常，在进行 NMP 之前，肾脏会先用器官保存液冲洗掉血液残留并冷却。在这里，我们评估了冷预充与直接 NMP 的效果。在 30 分钟的热缺血后，猪的肾脏要么先用冷组氨酸-色氨酸-酮戊二酸溶液进行预充（PFNMP 组），然后再用血液/缓冲液直接进行 NMP（DNMP 组）。NMP 在 75mmHg 的灌注压力下进行 6 小时。评估了功能参数以及组织病理学和生化分析。以肌酐清除率、钠排泄分数和尿液总量表示的肾功能在 PFNMP 中较差。尿液蛋白和中性粒细胞明胶酶相关脂质运载蛋白（NGAL）浓度作为肾脏损伤的标志物在 PFNMP 组中明显升高。此外，在 PFNMP 后还发现了渗透压肾病。这项研究表明，与直接进行热缺血损伤肾脏的 NMP 相比，在进行 NMP 之前进行冷预充会加重缺血再灌注损伤。随着 NMP 系统在肾脏和其他器官中的应用越来越多，有必要对采集过程中的移植物冲洗进行进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/ca28e4cfb810/41598_2019_50101_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/e2fa3fd5eded/41598_2019_50101_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/7342e90b0500/41598_2019_50101_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/9475b0077bf2/41598_2019_50101_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/2e9111e87dc7/41598_2019_50101_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/c27712d8cd8c/41598_2019_50101_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/47105c4db6a3/41598_2019_50101_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/5c40b8e3cbe8/41598_2019_50101_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/ca28e4cfb810/41598_2019_50101_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/e2fa3fd5eded/41598_2019_50101_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/7342e90b0500/41598_2019_50101_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/9475b0077bf2/41598_2019_50101_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/2e9111e87dc7/41598_2019_50101_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/c27712d8cd8c/41598_2019_50101_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/47105c4db6a3/41598_2019_50101_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/5c40b8e3cbe8/41598_2019_50101_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b4/6761287/ca28e4cfb810/41598_2019_50101_Fig8_HTML.jpg

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Am J Transplant. 2019 Mar;19(3):737-751. doi: 10.1111/ajt.15063. Epub 2018 Sep 17.
2
Improved approach for normothermic machine perfusion of cold stored kidney grafts.冷保存肾移植常温机器灌注的改进方法。
Am J Transl Res. 2018 Jun 15;10(6):1921-1929. eCollection 2018.
3
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Antioxidants (Basel). 2022 Jul 6;11(7):1329. doi: 10.3390/antiox11071329.
4
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Int J Mol Sci. 2022 Apr 30;23(9):4989. doi: 10.3390/ijms23094989.
5
Improved Normothermic Machine Perfusion After Short Oxygenated Hypothermic Machine Perfusion of Ischemically Injured Porcine Kidneys.在对缺血损伤的猪肾脏进行短时间充氧低温机器灌注后，常温机器灌注得到改善
Transplant Direct. 2021 Jan 15;7(2):e653. doi: 10.1097/TXD.0000000000001108. eCollection 2021 Feb.
6
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