Department of Pediatrics, Girona Institute for Biomedical Research (IDIBGI), Salt, Spain.
Department of Physical Therapy, EUSES University of Girona, Salt Girona, Spain.
Int J Obes (Lond). 2020 Jan;44(1):13-22. doi: 10.1038/s41366-019-0450-9. Epub 2019 Sep 25.
To study DNA methylation at the C19MC locus in the placenta and its association with (1) parental body size, (2) transmission of haplotypes for the C19MC rs55765443 SNP, and (3) offspring's body size and/or body composition at birth and in childhood.
Seventy-two pregnant women-infant pairs and 63 fathers were included in the study. Weight and height of mothers, fathers and newborns were registered during pregnancy or at birth (n = 72). Placental DNA methylation at the C19MC imprinting control region (ICR) was quantified by bisulfite pyrosequencing. Genotyping of the SNP was performed using restriction fragment length polymorphisms. The children's body size and composition were reassessed at age 6 years (n = 32).
Lower levels of placental C19MC methylation were associated with increased body size of mother, specifically with higher pregestational and predelivery weights and height of the mother (β from -0.294 to -0.371; R from 0.04 to 0.10 and all p < 0.019), and with higher weight, height, waist and hip circumferences, and fat mass of the child (β from -0.428 to -0.552; R from 0.33 to 0.56 and all p < 0.009). Parental transmission of the SNP did not correlate with an altered placental methylation status at the C19MC ICR.
Increased maternal size is associated with reduced placental C19MC methylation, which, in turn, relate to larger body size of the child.
研究胎盘 C19MC 基因座的 DNA 甲基化及其与（1）父母体型、（2）C19MC rs55765443 SNP 单倍型的传递和（3）后代出生时及儿童期的体型和/或身体成分的关系。
本研究纳入了 72 对孕妇-婴儿和 63 位父亲。母亲、父亲和新生儿在妊娠期间或出生时记录体重和身高（n=72）。采用亚硫酸氢盐焦磷酸测序法定量测定胎盘 C19MC 印迹控制区（ICR）的 DNA 甲基化。采用限制性片段长度多态性检测 SNP 的基因分型。在 6 岁时重新评估儿童的体型和成分（n=32）。
胎盘 C19MC 甲基化水平较低与母亲体型增大相关，具体表现为母亲孕前和产前体重及身高较高（β值为-0.294 至-0.371；R 值为 0.04 至 0.10，所有 p 值均<0.019），且儿童体重、身高、腰围和臀围以及脂肪量较高（β值为-0.428 至-0.552；R 值为 0.33 至 0.56，所有 p 值均<0.009）。SNP 的父母传递与 C19MC ICR 处胎盘甲基化状态的改变无关。
母亲体型增大与胎盘 C19MC 甲基化减少相关，而后者又与儿童体型增大相关。
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