Morningside Laboratory for Chemical Biology, Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.
Angew Chem Int Ed Engl. 2017 Oct 9;56(42):12873-12877. doi: 10.1002/anie.201705873. Epub 2017 Sep 13.
The hydroxyl radical ( OH), one of the most reactive and deleterious reactive oxygen species (ROS), has been suggested to play an essential role in many physiological and pathological scenarios. However, a reliable and robust method to detect endogenous OH is currently lacking owing to its extremely high reactivity and short lifetime. Herein we report a fluorescent probe HKOH-1 with superior in vitro selectivity and sensitivity towards OH. With this probe, we have calibrated and quantified the scavenging capacities of a wide range of reported OH scavengers. Furthermore, HKOH-1r, which was designed for better cellular uptake and retention, has performed robustly in detection of endogenous OH generation by both confocal imaging and flow cytometry. Furthermore, this probe has been applied to monitor OH generation in HeLa cells in response to UV light irradiation. Therefore, HKOH-1 could be used for elucidating OH related biological functions.
羟基自由基（OH）是最具反应性和有害的活性氧物种（ROS）之一，被认为在许多生理和病理情况下发挥着重要作用。然而，由于其极高的反应活性和短暂的寿命，目前仍然缺乏一种可靠和稳健的方法来检测内源性 OH。在此，我们报告了一种荧光探针 HKOH-1，它对 OH 具有优异的体外选择性和灵敏度。利用该探针，我们对广泛报道的 OH 清除剂的清除能力进行了校准和定量。此外，为了更好地进行细胞摄取和保留而设计的 HKOH-1r，在通过共聚焦成像和流式细胞术检测内源性 OH 生成方面表现出了稳健性。此外，该探针还被应用于监测 HeLa 细胞在紫外光照射下生成 OH。因此，HKOH-1 可用于阐明与 OH 相关的生物学功能。
