Gasser D L, Winters B A, Haas J B, McKearn T J, Kennett R H
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4636-40. doi: 10.1073/pnas.76.9.4636.
Spleen cells from a LEW.AVN rat immunized with cells from an MNR rat were fused with mouse myeloma cells to produce hybrid cell lines. One of these hybridomas produced a monoclonal antibody that was cytotoxic for bone marrow-derived (B) but not thymus-derived (T) cells. The antigen defined by this antibody is determined by a gene linked to the major histocompatibility complex (MHC). The antigen is also present on B cells of most mouse strains and is determined by an MHC-linked gene in this species as well. In both rats and mice, the gene determining the antigen maps within the immune response region of the MHC. All human B-cell lines, but not T-cell lines, and B but not T cells of all human donors tested so far are also positive for this antigen. Among human-mouse somatic cell lines that have lost various human chromosomes, this B-cell antigen is present on all lines that are positive for HLA antigen but is absent from all lines that have lost HLA.
用MNR大鼠的细胞免疫的LEW.AVN大鼠的脾细胞与小鼠骨髓瘤细胞融合，以产生杂交细胞系。其中一种杂交瘤产生了一种单克隆抗体，该抗体对骨髓来源的（B）细胞具有细胞毒性，但对胸腺来源的（T）细胞没有细胞毒性。该抗体所定义的抗原由一个与主要组织相容性复合体（MHC）连锁的基因决定。该抗原也存在于大多数小鼠品系的B细胞上，在该物种中同样由一个与MHC连锁的基因决定。在大鼠和小鼠中，决定该抗原的基因都定位在MHC的免疫反应区域内。所有人类B细胞系，而非T细胞系，以及迄今为止测试的所有人类供体的B细胞而非T细胞，对该抗原也呈阳性反应。在已经丢失各种人类染色体的人 - 小鼠体细胞系中，这种B细胞抗原存在于所有对HLA抗原呈阳性的细胞系中，但在所有已经丢失HLA的细胞系中不存在。
