Haynes B F, Eisenbarth G S, Fauci A S
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5829-33. doi: 10.1073/pnas.76.11.5829.
A monoclonal mouse antibody (3A1) that specifically bound to 65% of human peripheral blood (PB) thymus-derived (T) cells but did not bind to complement receptor-positive PB bone marrow-derived (B) cells, polymorphonuclear leukocytes, or human erythrocytes has been produced. The 3AI antibody was synthesized by a stable cloned lymphocyte hybrid cell line. This lymphocyte hybrid line (3AI) was derived from fusion of P3 X 63/Ag8 myeloma cells and spleen cells from BALB/c mice immunized with HSB-2 cells, a human T cell line. The 3A1 lymphocyte hybrid line produced mouse ascites fluid containing 3A1 antibody in saturating titers of up to 1:25,600. Purified PB T cells that carried the 3A1 antigen incorporated tritiated thymidine maximally in response to phytohemagglutinin and concanavalin A stimulation, whereas purified PB T cells that lacked the 3A1 antigen responded suboptimally to phytohemagglutinin and minimally to concanavalin A. Thus, the 3A1 antibody can be easily used to study the role of 3A1-positive and negative T cell subsets in the regulation of normal and abnormal human immune responses.
已制备出一种单克隆小鼠抗体（3A1），它能特异性结合65%的人外周血（PB）胸腺来源（T）细胞，但不与补体受体阳性的PB骨髓来源（B）细胞、多形核白细胞或人红细胞结合。3AI抗体由稳定的克隆淋巴细胞杂交细胞系合成。该淋巴细胞杂交系（3AI）源自P3 X 63/Ag8骨髓瘤细胞与用人类T细胞系HSB-2细胞免疫的BALB/c小鼠脾细胞的融合。3A1淋巴细胞杂交系产生的小鼠腹水含有3A1抗体，饱和效价高达1:25,600。携带3A1抗原的纯化PB T细胞在受到植物血凝素和刀豆球蛋白A刺激时，能最大程度地掺入氚标记胸腺嘧啶核苷，而缺乏3A1抗原的纯化PB T细胞对植物血凝素反应欠佳，对刀豆球蛋白A反应微弱。因此，3A1抗体可轻易用于研究3A1阳性和阴性T细胞亚群在正常和异常人类免疫反应调节中的作用。
