Sakurada K, Miyazaki T, Kimura K, Yamabe M, Katabami F, Koyama M, Uehara Y, Katoh N
Cancer Res. 1985 Feb;45(2):903-8.
Phospholipid-sensitive Ca2+-dependent protein kinase (PL-Ca-PK) and its substrates were investigated in neutrophils from normal subjects and in chronic myelocytic and acute myelocytic leukemic cells from patients with or without treatment for leukemia. PL-Ca-PK and its substrates were found in total particulate fraction of normal neutrophils, but less in cytosol. In leukemic cells from chronic myelocytic leukemia patients without treatment, PL-Ca-PK and its substrate, Mr 38,000 protein, increased in cytosol but decreased in total particulate fraction as compared with normal neutrophils. In leukemic cells obtained from chronic myelocytic leukemia patients after treatment mainly with busulfan, PL-Ca-PK and Mr 38,000 protein were increased in total particulate fraction but decreased in cytosol. Using leukemic cells from acute myelocytic leukemia patients with or without treatment, similar results were obtained. The change of localization of PL-Ca-PK and Mr 38,000 protein in leukemic cells appeared to be correlated to the increase or decrease of the number of leukemic cells. These results suggested that PL-Ca-PK together with the substrate, Mr 38,000 protein, might be translocated from total particulate fraction to cytosol with the onset of leukemia, and from cytosol to total particulate fraction accompanying treatment for leukemia.
在正常受试者的中性粒细胞以及白血病患者经或未经白血病治疗的慢性粒细胞白血病和急性粒细胞白血病细胞中，对磷脂敏感的钙依赖性蛋白激酶（PL-Ca-PK）及其底物进行了研究。在正常中性粒细胞的总颗粒部分中发现了PL-Ca-PK及其底物，但在胞质溶胶中较少。在未经治疗的慢性粒细胞白血病患者的白血病细胞中，与正常中性粒细胞相比，PL-Ca-PK及其底物（分子量38,000的蛋白质）在胞质溶胶中增加，但在总颗粒部分中减少。在主要用白消安治疗后的慢性粒细胞白血病患者获得的白血病细胞中，PL-Ca-PK和分子量38,000的蛋白质在总颗粒部分中增加，但在胞质溶胶中减少。使用急性粒细胞白血病患者经或未经治疗的白血病细胞，获得了类似的结果。白血病细胞中PL-Ca-PK和分子量38,000蛋白质的定位变化似乎与白血病细胞数量的增加或减少相关。这些结果表明，PL-Ca-PK与底物分子量38,000的蛋白质一起，可能在白血病发生时从总颗粒部分转移到胞质溶胶中，并在白血病治疗过程中从胞质溶胶转移到总颗粒部分。
