Kimura K, Katoh N, Sakurada K, Kubo S
Endocrinology. 1984 Dec;115(6):2391-9. doi: 10.1210/endo-115-6-2391.
The presence and localization in pig and rat testes of phospholipid-sensitive Ca2+-dependent protein kinase (PL-Ca-PK) and its substrates were investigated. PL-Ca-PK activity was found in the testis total particulate fraction and epididymal fluid, but little in the testis total particulate fraction and epididymal fluid, but little in the testis cytosol and matured spermatozoa obtained from the epididymis. Similarly, at least three endogenous substrates (83,000, 33,000, and 26,000) and five substrates (greater than 100,000, 83,000, 60,000, 43,000, and 19,000) for PL-Ca-PK were detected in the testis total particulate fraction and the epididymal fluid, respectively, but little or no substrates were observed in the testis cytosol and matured spermatozoa. The three substrates detected in the total particulate fraction were also observed in the testis nuclear fraction. In rat testis, PL-Ca-PK activity was detected in the total particulate fraction of germ cells. The results suggested that PL-Ca-PK system might be important in membrane- or subcellular organellar-associated functions in testis.
研究了磷脂敏感的钙依赖性蛋白激酶（PL-Ca-PK）及其底物在猪和大鼠睾丸中的存在情况及定位。在睾丸总微粒体部分和附睾液中发现了PL-Ca-PK活性，但在睾丸总微粒体部分和附睾液中活性较低，而在睾丸胞质溶胶和从附睾获得的成熟精子中活性很低。同样，在睾丸总微粒体部分和附睾液中分别检测到至少三种PL-Ca-PK的内源性底物（83,000、33,000和26,000）和五种底物（大于100,000、83,000、60,000、43,000和19,000），但在睾丸胞质溶胶和成熟精子中几乎未观察到底物。在总微粒体部分检测到的三种底物在睾丸核部分也有观察到。在大鼠睾丸中，在生殖细胞的总微粒体部分检测到PL-Ca-PK活性。结果表明，PL-Ca-PK系统可能在睾丸中与膜或亚细胞器相关的功能中起重要作用。
