Koizumi H, Aoyagi T, Ohkawara A
Arch Dermatol Res. 1986;279(2):95-9. doi: 10.1007/BF00417529.
Endogenous calcium-activated, phospholipid-dependent protein kinase phosphorylates pig epidermal protein. Pig epidermis was homogenized and centrifuged at 30,000 X g for 30 min. The supernatant was incubated with or without calcium and phospholipid. A 97 kD soluble protein from pig epidermis was phosphorylated in the presence of calcium and phosphatidylserine. The phosphorylation reaction occurred immediately. With the use of two-dimensional polyacrylamide gel electrophoresis, it was shown that the 97 kD fragment was a basic protein, and that several small proteins were also phosphorylated. The characterization of these proteins is yet to be undertaken.
内源性钙激活的磷脂依赖性蛋白激酶使猪表皮蛋白发生磷酸化。将猪表皮匀浆并在30,000×g下离心30分钟。将上清液在有或无钙和磷脂的情况下进行孵育。来自猪表皮的一种97kD可溶性蛋白在钙和磷脂酰丝氨酸存在的情况下发生磷酸化。磷酸化反应立即发生。使用二维聚丙烯酰胺凝胶电泳表明，97kD片段是一种碱性蛋白，并且还有几种小蛋白也发生了磷酸化。这些蛋白的特性还有待确定。
