Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, People's Republic of China.
Food Funct. 2019 Oct 16;10(10):6699-6710. doi: 10.1039/c9fo00654k.
Endometritis is an inflammatory disease of the uterus caused by bacterial infection, and it affects both human and animal health. This study aims to investigate the protective effects and molecular mechanisms of probiotics such as Clostridium tyrobutyricum (C. tyrobutyricum) on Staphylococcus aureus (S. aureus)-induced endometritis. The results showed that S. aureus infection significantly induced the pathological damage of the uterus, increased the production of pro-inflammatory cytokines, such as TNF-α and IL-1β, and attenuated the expression of tight junction proteins of uterine tissues. However, C. tyrobutyricum pretreatment obviously reduced the inflammatory response and reversed the changes of tight junction proteins of the uterus induced by S. aureus. Together, the data showed that C. tyrobutyricum also inhibited the expression of the TLR2/NF-κB signaling pathway and HDAC induced by S. aureus. In addition, the treatment of mice with live C. tyrobutyricum, spent culture supernatants (SCS) from C. tyrobutyricum, rather than inactive C. tyrobutyricum, inhibited the inflammatory response induced by S. aureus. Through further research, we found that the levels of butyrate in both blood and uterine tissues of mice treated with C. tyrobutyricum were significantly increased. These findings underscore the protective effect of C. tyrobutyricum on endometritis by enhancing the uterus barrier integrity and inhibiting the inflammatory response. The anti-inflammatory mechanism may occur through the regulation of the expression of TLR2/NF-κB and HDAC, and C. tyrobutyricum can be a potentially therapeutic candidate for the treatment of endometritis.
子宫内膜炎是一种由细菌感染引起的子宫炎症性疾病，影响人类和动物的健康。本研究旨在探讨丁酸梭菌（C. tyrobutyricum）等益生菌对金黄色葡萄球菌（S. aureus）诱导的子宫内膜炎的保护作用及其分子机制。结果表明，金黄色葡萄球菌感染显著诱导了子宫的病理损伤，增加了促炎细胞因子如 TNF-α和 IL-1β的产生，并减弱了子宫组织紧密连接蛋白的表达。然而，丁酸梭菌预处理明显减轻了金黄色葡萄球菌诱导的炎症反应，并逆转了子宫紧密连接蛋白的变化。总之，数据表明丁酸梭菌还抑制了金黄色葡萄球菌诱导的 TLR2/NF-κB 信号通路和组蛋白去乙酰化酶（HDAC）的表达。此外，用活丁酸梭菌、丁酸梭菌的废弃培养上清液（SCS）而非失活丁酸梭菌处理小鼠，可抑制金黄色葡萄球菌诱导的炎症反应。通过进一步研究，我们发现用丁酸梭菌处理的小鼠的血液和子宫组织中的丁酸盐水平均显著增加。这些发现强调了丁酸梭菌通过增强子宫屏障完整性和抑制炎症反应对子宫内膜炎的保护作用。抗炎机制可能通过调节 TLR2/NF-κB 和 HDAC 的表达来发生，丁酸梭菌可能是治疗子宫内膜炎的潜在治疗候选药物。
