IFN- Displays Anti-Inflammatory Effects on Endometritis via Inhibiting the Activation of the NF-B and MAPK Pathways in Mice.

The aim of the present study was to determine the anti-inflammatory effect of IFN- on endometritis using a mouse model of -induced endometritis and to elucidate the mechanism of action underlying these effects. In the present study, the effect of IFN- on growth was monitored by turbidimeter at 600 nm. IFN- did not affect growth. The histopathological changes indicated that IFN- had a protective effect on uterus tissues with infection. The ELISA and qPCR results showed the production of the proinflammatory cytokines TNF-, IL-1, and IL-6 was decreased with IFN- treatment. In contrast, the level of the anti-inflammatory cytokine IL-10 was increased. We further studied the signaling pathway associated with these observations, and the qPCR results showed that the expression of TLR2 was repressed by IFN-. Furthermore, the western blotting results showed the phosphorylation of IB, NF-B p65, and MAPKs (p38, JNK, and ERK) was inhibited by IFN- treatment. The results suggested that IFN- may be a potential drug for the treatment of uterine infection due to or other infectious inflammatory diseases.