原噬菌体λ突变体诱导效率与recA等位基因的关系。
Efficiency of induction of prophage lambda mutants as a function of recA alleles.
作者信息
Dutreix M, Bailone A, Devoret R
出版信息
J Bacteriol. 1985 Mar;161(3):1080-5. doi: 10.1128/jb.161.3.1080-1085.1985.
Abstract
Mutants of the cI gene of prophage lambda have been defined phenotypically in a recA+ host as noninducible (Ind-), inducible (Ind+), or induction sensitive (Inds). We showed that a phage lambda cI+ carrying operator mutations v2 and v3 displays an Inds phenotype, as does lambda cI inds-1. We characterized a fourth induction phenotype called induction resistant (Indr). Using these four prophage types, we tested the influence of bacterial recA mutations on prophage induction. Indr prophages were fully induced in recA441 bacteria whose RecA441 protein is activated constitutively. Indr prophages were not induced in a mutant overproducing RecA+ protein, confirming that RecA+ protein must be activated to promote prophage induction. Inds prophages were induced in recA142 and recA453-441 lysogens, previously described as deficient in prophage induction.
摘要
原噬菌体λ的cI基因突变体在recA⁺宿主中已被表型定义为不可诱导型（Ind⁻）、可诱导型（Ind⁺）或诱导敏感型（Inds）。我们发现携带操纵基因突变v2和v3的噬菌体λ cI⁺表现出Inds表型，λ cI inds⁻1也是如此。我们鉴定出了第四种诱导表型，称为诱导抗性型（Indr）。利用这四种原噬菌体类型，我们测试了细菌recA突变对原噬菌体诱导的影响。Indr原噬菌体在RecA441蛋白被组成型激活的recA441细菌中能被完全诱导。Indr原噬菌体在过量产生RecA⁺蛋白的突变体中未被诱导，这证实了RecA⁺蛋白必须被激活才能促进原噬菌体诱导。Inds原噬菌体在recA142和recA453 - 441溶原菌中被诱导，这两种溶原菌先前被描述为原噬菌体诱导缺陷型。
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