噬菌体λ阻遏物中阻止RecA介导切割的突变。
Mutations in bacteriophage lambda repressor that prevent RecA-mediated cleavage.
作者信息
Gimble F S, Sauer R T
出版信息
J Bacteriol. 1985 Apr;162(1):147-54. doi: 10.1128/jb.162.1.147-154.1985.
Abstract
In this paper, we report on the isolation and sequence analysis of mutations that confer an induction-deficient phenotype to lambda repressor. A total of 16 different mutations, which occur at 13 different sites in the repressor gene, have been characterized. For most of the mutant lysogens, frequencies of spontaneous induction in a recA+ strain were reduced dramatically in comparison with those for a wild-type phage, and these mutant lysogens showed little or no prophage induction after UV irradiation. The immunity properties of cells containing the mutant repressors show that all of the mutants but one exhibit operator-binding properties indistinguishable from wild-type repressor.
摘要
在本文中，我们报告了赋予λ阻遏物诱导缺陷表型的突变的分离和序列分析。总共16种不同的突变已被鉴定，这些突变发生在阻遏物基因的13个不同位点。对于大多数突变溶原菌，与野生型噬菌体相比，recA+菌株中自发诱导的频率显著降低，并且这些突变溶原菌在紫外线照射后几乎没有或没有原噬菌体诱导。含有突变阻遏物的细胞的免疫特性表明，除了一个突变体之外，所有突变体都表现出与野生型阻遏物无法区分的操纵子结合特性。
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