Faculty of Dentistry, Universidad Cooperativa de Colombia. Carrera 47 # 37 sur 18, Envigado, Colombia.
Production Engineering Department, Universidad Eafit, Carrera 49 # 7 sur 50, Medellín, Colombia.
Arch Oral Biol. 2020 Jan;109:104573. doi: 10.1016/j.archoralbio.2019.104573. Epub 2019 Sep 24.
The purpose of this investigation was to establish microstructure, microhardness, fracture toughness, chemical composition, and crack repair of bovine enamel and to compare these features with their human counterparts.
Bovine enamel fragments were prepared and optical microscopy and atomic force microscopy were used to establish microstructure; Raman spectroscopy was used to estimate composition and microindentation using Vickers testing was performed to evaluate hardness.
A strong dependence between indentation load and microhardness values was observed, as was the case in human enamel. Similar microstructure and chemical composition between bovine and human enamel, 7.89% lower microhardness and 40% higher fracture toughness values for bovine enamel were found.
From a structural and mechanical standpoint, bovine enamel is a suitable alternative to human enamel for in vitro testing of dental products.
本研究旨在建立牛牙釉质的微观结构、显微硬度、断裂韧性、化学成分和裂纹修复，并将这些特征与人类牙釉质进行比较。
制备牛牙釉质碎片，采用光学显微镜和原子力显微镜建立微观结构；拉曼光谱用于估计成分，维氏硬度测试用于微压痕硬度评估。
观察到压痕载荷与显微硬度值之间存在很强的依赖性，这与人类牙釉质的情况相同。发现牛牙釉质与人类牙釉质具有相似的微观结构和化学成分，但显微硬度低 7.89%，断裂韧性高 40%。
从结构和力学角度来看，牛牙釉质是体外牙釉质检测替代人类牙釉质的合适选择。
