College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China; Hunan International Scientific and Technological Cooperation Base of Agricultural Typical Pollution Remediation and Wetland Protection, Hunan Agricultural University, Changsha, 410028, China.
College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China; Hunan International Scientific and Technological Cooperation Base of Agricultural Typical Pollution Remediation and Wetland Protection, Hunan Agricultural University, Changsha, 410028, China; Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
J Environ Manage. 2019 Dec 1;251:109542. doi: 10.1016/j.jenvman.2019.109542. Epub 2019 Sep 27.
Microbial community is sensitive to the variations of environment, and it plays an important role in biogeochemical cycling in acid mine drainage (AMD). In this study, an integrated high-throughput absolute abundance quantification (iHAAQ) method was applied to study the dynamics of microbial community and the characteristics of microorganism. The results showed a significant difference in bacterial community with diversity being higher in watershed area. The main influential factors for bacterial communities in watershed were physicochemical properties (e.g., pH and potassium), while in mining areas the main driving factors were metals/metalloids (e.g., As, Zn, and Pb). Notably, the major functions of microbial community were transporter and ABC transporter in mining area, while two-component system was more abundant in watershed by the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis (level 3). In particular, Phyllobacterium, Bacteroides, and Sulfurovum were demonstrated to be potentially useful bacterial species for bioremediation, which should be a good choice for future studies. These results could facilitate our understanding of microbial diversity in different sediments of mining areas and identify microbial communities for bioremediation projects.
微生物群落对环境变化敏感，在酸性矿山排水（AMD）中的生物地球化学循环中起着重要作用。本研究采用集成高通量绝对定量（iHAAQ）方法研究了微生物群落的动态变化和微生物特征。结果表明，流域区的细菌群落多样性较高，存在显著差异。影响流域区细菌群落的主要因素是理化性质（如 pH 值和钾），而在矿区，主要驱动因素是金属/类金属（如 As、Zn 和 Pb）。值得注意的是，通过京都基因与基因组百科全书（KEGG）注释分析（第 3 级），矿区微生物群落的主要功能是转运蛋白和 ABC 转运蛋白，而流域区的主要功能是二组分系统。特别是， Phyllobacterium、Bacteroides 和 Sulfurovum 被证明是生物修复中潜在有用的细菌物种，这将是未来研究的一个很好的选择。这些结果可以帮助我们了解矿区不同沉积物中的微生物多样性，并确定生物修复项目中的微生物群落。
