质谱分析揭示了通过氧化聚丙烯废料片段生物转化获得的聚羟基脂肪酸酯的分子结构。

Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments.

作者信息

Johnston Brian, Radecka Iza, Chiellini Emo, Barsi David, Ilieva Vassilka Ivanova, Sikorska Wanda, Musioł Marta, Zięba Magdalena, Chaber Paweł, Marek Adam A, Mendrek Barbara, Ekere Anabel Itohowo, Adamus Grazyna, Kowalczuk Marek

机构信息

Wolverhampton School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UK.

Laboratorio Materiali Polimerici Ecocompatibili (LMPE), via Nuova, 44/a, Segromigno in Monte, 55018 Capannori (LU), Italy.

出版信息

Polymers (Basel). 2019 Sep 27;11(10):1580. doi: 10.3390/polym11101580.

This study investigated the molecular structure of the polyhydroxyalkanoate (PHA) produced via a microbiological shake flask experiment utilizing oxidized polypropylene (PP) waste as an additional carbon source. The bacterial strain H16 was selected as it is non-pathogenic, genetically stable, robust, and one of the best known producers of PHA. Making use of PHA oligomers, formed by controlled moderate-temperature degradation induced by carboxylate moieties, by examination of both the parent and fragmentation ions, the ESI-MS/MS analysis revealed the 3-hydroxybutyrate and randomly distributed 3-hydroxyvalerate as well as 3-hydroxyhexanoate repeat units. Thus, the bioconversion of PP solid waste to a value-added product such as PHA tert-polymer was demonstrated.