• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

废弃利乐包装中聚乙烯向聚羟基脂肪酸酯的生物转化过程

Bioconversion Process of Polyethylene from Waste Tetra Pak Packaging to Polyhydroxyalkanoates.

作者信息

Ekere Itohowo, Johnston Brian, Tchuenbou-Magaia Fideline, Townrow David, Wojciechowski Szymon, Marek Adam, Zawadiak Jan, Duale Khadar, Zieba Magdalena, Sikorska Wanda, Adamus Grazyna, Goslar Tomasz, Kowalczuk Marek, Radecka Iza

机构信息

School of Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UK.

Science in Industry Research Centre (SIRC), SciTech Innovation Hub, Wolverhampton Science Park, Glaisher Drive, Wolverhampton WV10 9RU, UK.

出版信息

Polymers (Basel). 2022 Jul 12;14(14):2840. doi: 10.3390/polym14142840.

DOI:10.3390/polym14142840
PMID:35890616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9317417/
Abstract

Presented herein are the results of a novel recycling method for waste Tetra Pak packaging materials. The polyethylene (PE-T) component of this packaging material, obtained via a separation process using a "solvents method", was used as a carbon source for the biosynthesis of polyhydroxyalkanoates (PHAs) by the bacterial strain H16. Bacteria were grown for 48-72 h, at 30 °C, in TSB (nitrogen-rich) or BSM (nitrogen-limited) media supplemented with PE-T. Growth was monitored by viable counting. It was demonstrated that utilised PE-T in both growth media, but was only able to accumulate 40% PHA in TSB supplemented with PE-T. Only 1.5% PHA was accumulated in the TSB control, and no PHA was detected in the BSM control. Extracted biopolymers were characterised by nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR) spectroscopy, electrospray tandem mass spectrometry (ESI-MS/MS), gel permeation chromatography (GPC), and accelerator mass spectrometry (AMS). The characterisation of PHA by ESI-MS/MS revealed that PHA produced by in TSB supplemented with PE-T contained 3-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate co-monomeric units. AMS analysis also confirmed the presence of 96.73% modern carbon and 3.27% old carbon in PHA derived from Tetra Pak. Thus, this study demonstrates the feasibility of our proposed recycling method for waste Tetra Pak packaging materials, alongside its potential for producing value-added PHA, and the ability of 14C analysis in validating this bioconversion process.

摘要

本文展示了一种针对废弃利乐包装材料的新型回收方法的结果。通过“溶剂法”分离过程获得的这种包装材料的聚乙烯（PE-T）成分，被用作细菌菌株H16生物合成聚羟基脂肪酸酯（PHA）的碳源。细菌在30°C下于补充有PE-T的TSB（富氮）或BSM（氮限制）培养基中培养48 - 72小时。通过活菌计数监测生长情况。结果表明，在两种生长培养基中均利用了PE-T，但在补充有PE-T的TSB中仅能积累40%的PHA。在TSB对照中仅积累了1.5%的PHA，而在BSM对照中未检测到PHA。提取的生物聚合物通过核磁共振（NMR）、傅里叶变换红外（FTIR）光谱、电喷雾串联质谱（ESI-MS/MS）、凝胶渗透色谱（GPC）和加速器质谱（AMS）进行表征。通过ESI-MS/MS对PHA的表征表明，在补充有PE-T的TSB中由该菌株产生的PHA含有3-羟基丁酸、3-羟基戊酸和3-羟基己酸共聚单体单元。AMS分析还证实，源自利乐包装的PHA中存在96.73%的现代碳和3.27%的古老碳。因此，本研究证明了我们提出的废弃利乐包装材料回收方法的可行性，以及其生产增值PHA的潜力，以及14C分析在验证这种生物转化过程中的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/fd3baba28eea/polymers-14-02840-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/595f19dec8bb/polymers-14-02840-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/cc945fceabb7/polymers-14-02840-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/ddb6b73cf047/polymers-14-02840-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/3f01396dfbbb/polymers-14-02840-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/732162d2fdda/polymers-14-02840-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/b8da98ccf30f/polymers-14-02840-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/6314983c8586/polymers-14-02840-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/bece98ff63f5/polymers-14-02840-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/fd3baba28eea/polymers-14-02840-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/595f19dec8bb/polymers-14-02840-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/cc945fceabb7/polymers-14-02840-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/ddb6b73cf047/polymers-14-02840-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/3f01396dfbbb/polymers-14-02840-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/732162d2fdda/polymers-14-02840-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/b8da98ccf30f/polymers-14-02840-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/6314983c8586/polymers-14-02840-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/bece98ff63f5/polymers-14-02840-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9983/9317417/fd3baba28eea/polymers-14-02840-g009.jpg

相似文献

1
Bioconversion Process of Polyethylene from Waste Tetra Pak Packaging to Polyhydroxyalkanoates.废弃利乐包装中聚乙烯向聚羟基脂肪酸酯的生物转化过程
Polymers (Basel). 2022 Jul 12;14(14):2840. doi: 10.3390/polym14142840.
2
Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production.氧化聚乙烯蜡作为聚羟基脂肪酸酯（PHA）生产的潜在碳源。
Materials (Basel). 2016 May 13;9(5):367. doi: 10.3390/ma9050367.
3
Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments.质谱分析揭示了通过氧化聚丙烯废料片段生物转化获得的聚羟基脂肪酸酯的分子结构。
Polymers (Basel). 2019 Sep 27;11(10):1580. doi: 10.3390/polym11101580.
4
The Microbial Production of Polyhydroxyalkanoates from Waste Polystyrene Fragments Attained Using Oxidative Degradation.利用氧化降解从废弃聚苯乙烯碎片中微生物生产聚羟基脂肪酸酯
Polymers (Basel). 2018 Aug 29;10(9):957. doi: 10.3390/polym10090957.
5
The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production.以非氧化聚乙烯蜡为聚羟基脂肪酸酯生产的碳源，对源自聚乙烯的可生物降解聚合物进行分子水平表征。
Bioengineering (Basel). 2017 Aug 28;4(3):73. doi: 10.3390/bioengineering4030073.
6
Bioconversion of Plastic Waste Based on Mass Full Carbon Backbone Polymeric Materials to Value-Added Polyhydroxyalkanoates (PHAs).基于全碳主链聚合物材料的塑料废弃物生物转化为增值聚羟基脂肪酸酯（PHA）。
Bioengineering (Basel). 2022 Sep 1;9(9):432. doi: 10.3390/bioengineering9090432.
7
Chicken feather hydrolysate as an inexpensive complex nitrogen source for PHA production by Cupriavidus necator on waste frying oils.鸡毛水解物作为一种廉价的复合氮源，用于食油假单胞菌利用废煎炸油生产聚羟基脂肪酸酯。
Lett Appl Microbiol. 2017 Aug;65(2):182-188. doi: 10.1111/lam.12762.
8
A feeding strategy for incorporation of canola derived medium-chain-length monomers into the PHA produced by wild-type Cupriavidus necator.一种将油菜籽衍生的中链长度单体掺入野生型食酸铜绿假单胞菌产生的聚羟基脂肪酸酯中的进料策略。
World J Microbiol Biotechnol. 2014 Apr;30(4):1409-16. doi: 10.1007/s11274-013-1563-2. Epub 2013 Nov 28.
9
Polyhydroxyalkanoates production with Ralstonia eutropha from low quality waste animal fats.利用真养产碱杆菌从低质量废弃动物脂肪中生产聚羟基脂肪酸酯
J Biotechnol. 2015 Nov 20;214:119-27. doi: 10.1016/j.jbiotec.2015.09.002. Epub 2015 Sep 30.
10
Heterologous expression of phaC2 gene and poly-3-hydroxyalkanoate production by recombinant Cupriavidus necator strains using canola oil as carbon source.利用油菜籽油作为碳源，通过重组恶臭假单胞菌菌株实现phaC2 基因的异源表达和聚-3-羟基烷酸的生产。
N Biotechnol. 2018 Jan 25;40(Pt B):200-206. doi: 10.1016/j.nbt.2017.08.001. Epub 2017 Aug 19.

引用本文的文献

1
Experimental and Computational Insights into Polyurethane Plastic Waste Conversion to Microbial Bioplastic.聚氨酯塑料废料转化为微生物生物塑料的实验与计算见解
Curr Microbiol. 2025 Apr 3;82(5):227. doi: 10.1007/s00284-025-04218-w.
2
Bottlenecks in biobased approaches to plastic degradation.生物基方法在塑料降解方面的瓶颈。
Nat Commun. 2024 Jun 3;15(1):4715. doi: 10.1038/s41467-024-49146-8.
3
Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using ATCC 14581.利用ATCC 14581从木质纤维素废料生产聚（3-羟基丁酸酯）

本文引用的文献

1
Recovery, separation and production of fuel, plastic and aluminum from the Tetra PAK waste to hydrothermal and pyrolysis processes.从 Tetra PAK 废物中通过水热法和热解过程回收、分离和生产燃料、塑料和铝。
Waste Manag. 2022 Jan 1;137:179-189. doi: 10.1016/j.wasman.2021.11.007. Epub 2021 Nov 15.
2
Upcycling polyamide containing post-consumer Tetra Pak carton packaging to valuable chemicals and recyclable polymer.将含有消费后 Tetra Pak 纸盒包装的聚酰胺升级再造为有价值的化学品和可回收聚合物。
Waste Manag. 2021 Jul 15;131:423-432. doi: 10.1016/j.wasman.2021.06.031. Epub 2021 Jul 9.
3
Interaction effect of LED color temperatures and light-protective additive packaging on photo-oxidation in milk displayed in retail dairy case.
Polymers (Basel). 2023 Nov 22;15(23):4488. doi: 10.3390/polym15234488.
4
Matrix-Assisted Laser Desorption and Electrospray Ionization Tandem Mass Spectrometry of Microbial and Synthetic Biodegradable Polymers.微生物和合成可生物降解聚合物的基质辅助激光解吸和电喷雾电离串联质谱分析
Polymers (Basel). 2023 May 18;15(10):2356. doi: 10.3390/polym15102356.
5
Genotypic and Phenotypic Detection of Polyhydroxyalkanoate Production in Bacterial Isolates from Food.食品中细菌分离物中聚羟基烷酸生产的基因型和表型检测。
Int J Mol Sci. 2023 Jan 8;24(2):1250. doi: 10.3390/ijms24021250.
6
Bioconversion of Plastic Waste Based on Mass Full Carbon Backbone Polymeric Materials to Value-Added Polyhydroxyalkanoates (PHAs).基于全碳主链聚合物材料的塑料废弃物生物转化为增值聚羟基脂肪酸酯（PHA）。
Bioengineering (Basel). 2022 Sep 1;9(9):432. doi: 10.3390/bioengineering9090432.
零售乳制品冷藏柜中展示的牛奶，LED色温与光防护添加剂包装对其光氧化的交互作用。
Food Chem. 2020 Apr 3;323:126699. doi: 10.1016/j.foodchem.2020.126699.
4
Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments.质谱分析揭示了通过氧化聚丙烯废料片段生物转化获得的聚羟基脂肪酸酯的分子结构。
Polymers (Basel). 2019 Sep 27;11(10):1580. doi: 10.3390/polym11101580.
5
Efficacy of light-protective additive packaging in protecting milk freshness in a retail dairy case with LED lighting at different light intensities.不同光强 LED 照明下，光保护添加剂包装对零售奶品陈列柜中牛奶保鲜效果的影响。
Food Res Int. 2018 Dec;114:1-9. doi: 10.1016/j.foodres.2018.07.053. Epub 2018 Jul 31.
6
Cellulose I and II nanocrystals produced by sulfuric acid hydrolysis of Tetra pak cellulose I.由 Tetra pak 纤维素 I 硫酸水解得到的纤维素 I 和 II 纳米晶体。
Carbohydr Polym. 2018 Jul 15;192:184-192. doi: 10.1016/j.carbpol.2018.03.042. Epub 2018 Mar 18.
7
Changing Tetra Pak: from waste to resource.变革利乐包装：变废为宝。
Sci Prog. 2018 Jun 1;101(2):161-170. doi: 10.3184/003685018X15215434299329. Epub 2018 Apr 20.
8
The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production.以非氧化聚乙烯蜡为聚羟基脂肪酸酯生产的碳源，对源自聚乙烯的可生物降解聚合物进行分子水平表征。
Bioengineering (Basel). 2017 Aug 28;4(3):73. doi: 10.3390/bioengineering4030073.
9
Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production.氧化聚乙烯蜡作为聚羟基脂肪酸酯（PHA）生产的潜在碳源。
Materials (Basel). 2016 May 13;9(5):367. doi: 10.3390/ma9050367.
10
Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate.将消费后聚乙烯转化为可生物降解聚合物聚羟基脂肪酸酯。
Appl Microbiol Biotechnol. 2014 May;98(9):4223-32. doi: 10.1007/s00253-013-5489-2. Epub 2014 Jan 12.