Lee Juho, Hlaing Shwe Phyu, Cao Jiafu, Hasan Nurhasni, Ahn Hye-Jin, Song Ki-Won, Yoo Jin-Wook
College of Pharmacy, Pusan National University, Busan 46241, Korea.
Department of Organic Material Science and Engineering, Pusan National University, Busan 46241, Korea.
Pharmaceutics. 2019 Sep 27;11(10):496. doi: 10.3390/pharmaceutics11100496.
The eradication of bacteria from wound sites and promotion of healing are essential for treating infected wounds. Nitric oxide (NO) is desirable for these purposes due to its ability to accelerate wound healing and its broad-spectrum antibacterial effects. We developed an in situ hydrogel-forming/NO-releasing powder dressing (NO/GP), which is a powder during storage and forms a hydrogel when applied to wounds, as a novel NO-releasing formulation to treat infected wounds. An NO/GP fine powder (51.5 μm) was fabricated by blending and micronizing S-nitrosoglutathione (GSNO), alginate, pectin, and polyethylene glycol (PEG). NO/GP remained stable for more than four months when stored at 4 or 37 °C. When applied to wounds, NO/GP absorbed wound fluid and immediately converted to a hydrogel. Additionally, wound fluid triggered a NO release from NO/GP for more than 18 h. The rheological properties of hydrogel-transformed NO/GP indicated that NO/GP possesses similar adhesive properties to marketed products (Vaseline). NO/GP resulted in a 6-log reduction in colony forming units (CFUs) of methicillin resistant (MRSA) and which are representative drug-resistant gram-positive and -negative bacteria, respectively. The promotion of wound healing by NO/GP was demonstrated in mice with full-thickness wounds challenged with MRSA and . Thus, NO/GP is a promising formulation for the treatment of infected wounds.
从伤口部位清除细菌并促进愈合对于治疗感染伤口至关重要。一氧化氮（NO）因其能够加速伤口愈合和具有广谱抗菌作用而适用于这些目的。我们开发了一种原位水凝胶形成/NO释放粉末敷料（NO/GP），它在储存期间为粉末状，应用于伤口时形成水凝胶，作为一种治疗感染伤口的新型NO释放制剂。通过将S-亚硝基谷胱甘肽（GSNO）、藻酸盐、果胶和聚乙二醇（PEG）混合并微粉化制备了一种NO/GP细粉（51.5μm）。NO/GP在4℃或37℃储存时可保持稳定超过四个月。当应用于伤口时，NO/GP吸收伤口渗出液并立即转变为水凝胶。此外，伤口渗出液促使NO从NO/GP中释放超过18小时。水凝胶转变后的NO/GP的流变学性质表明，NO/GP具有与市售产品（凡士林）相似的粘附特性。NO/GP使耐甲氧西林金黄色葡萄球菌（MRSA）和（分别为代表性的耐药革兰氏阳性和阴性细菌）的菌落形成单位（CFU）减少了6个对数级。在受到MRSA和挑战的全层伤口小鼠中证明了NO/GP对伤口愈合的促进作用。因此，NO/GP是一种治疗感染伤口的有前景的制剂。
