Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.
Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy; PhD Program in Drug Discovery and Development, University of Salerno, via Giovanni Paolo II, 132, I-84084 Fisciano, SA, Italy.
Carbohydr Polym. 2020 Jan 1;227:115305. doi: 10.1016/j.carbpol.2019.115305. Epub 2019 Sep 9.
In this paper, alginate-pectin blend particles loaded with Annexin A1 derived peptide Ac2-26 as an in situ forming dressing was successfully developed for wound repair applications. High mannuronic (M) content alginate and amidated pectin blend have been used to encapsulate Ac2-26 in order to enhance stability of the peptide at room temperature and to control its release through the in situ formed gel. Ac2-26 recovery and FTIR studies suggests chemical interactions between peptide and polysaccharides blend able to improve the encapsulation efficiency of Ac2-26 into the polymer matrix and control its release, till 48 h. In vitro wound healing assay on HaCaT cells highlights the ability of Ac2-26 to significantly accelerate wound healing compared to unloaded particles, with complete closure of the wound model in 24 h. Therefore, all these results suggest that Ac2-26 loaded submicrometric in situ gelling powders could be a promising wound dressing to improve wound care armamentarium.
本文成功开发了一种载有 Annexin A1 衍生肽 Ac2-26 的海藻酸盐-果胶混合颗粒的原位形成敷料，用于伤口修复应用。高甘露糖（M）含量的海藻酸盐和酰胺化果胶的混合物被用来包裹 Ac2-26，以增强肽在室温下的稳定性，并通过原位形成的凝胶来控制其释放。Ac2-26 的回收和傅里叶变换红外（FTIR）研究表明，肽和多糖混合物之间的化学相互作用能够提高 Ac2-26 被包埋到聚合物基质中的包封效率，并控制其释放，直到 48 小时。在 HaCaT 细胞上的体外伤口愈合试验表明，与未加载的颗粒相比，Ac2-26 能够显著加速伤口愈合，在 24 小时内完全闭合伤口模型。因此，所有这些结果表明，载有 Ac2-26 的亚微米原位凝胶粉末可能是一种有前途的伤口敷料，以改善伤口护理手段。
