伊朗西北部动物源食品中产肠毒素和肠致病性分离株的监测。
Surveillance for enterotoxigenic & enteropathogenic isolates from animal source foods in Northwest Iran.
机构信息
Food & Drug Safety Research Center, Health Management & Safety Promotion Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Food Hygiene, Biotechnology Research Center, Tabriz Branch, Tehran, Iran.
出版信息
Indian J Med Res. 2019 Jul;150(1):87-91. doi: 10.4103/ijmr.IJMR_2019_17.
BACKGROUND & OBJECTIVES: Diarrhoeagenic Escherichia coli strains are common agents of diarrhoea particularly in developing countries. Food products of animal origin are considered as common carriers of E. coli. This study was undertaken to identify enterotoxigenic Escherichia coli (ETEC) and enteropathogenic E. coli (EPEC) pathotypes in animal-source foods (ASF).
METHODS
A total of 222 ASF samples were investigated. Based on the culture and biochemical tests, 109 E. coli isolates were identified. Duplex-polymerase chain reaction assay was used to detect ETEC and EPEC. The target genes selected for each category were the lt and st for the ETEC, and eae and bfp for the EPEC isolates.
RESULTS
The occurrence of E. coli in dairy and meat products was 45 and 52.5 per cent, respectively. Among the E. coli isolates, two ETEC, one typical EPEC and three atypical EPEC were detected in meat samples, whereas only one typical EPEC and one atypical EPEC were detected in dairy samples.
INTERPRETATION & CONCLUSIONS: Our results showed presence of ETEC and EPEC strains in ASFs. The milk without pasteurization and traditional dairy products produced in unhygienic conditions are most likely the main sources of E. coli pathotypes and other zoonotic pathogens and thus can be considered a potential hazard to the health of the community.
背景与目的
产肠毒性大肠杆菌菌株是发展中国家腹泻的常见病原体。动物源食品被认为是大肠杆菌的常见载体。本研究旨在鉴定动物源食品（ASF）中的肠产毒性大肠杆菌（ETEC）和肠致病性大肠杆菌（EPEC）血清型。
方法
共检测了 222 份 ASF 样本。根据培养和生化试验，鉴定出 109 株大肠杆菌分离株。采用双重聚合酶链反应检测 ETEC 和 EPEC。针对每种类别选择的靶基因是 ETEC 的 lt 和 st，以及 EPEC 分离株的 eae 和 bfp。
结果
乳制品和肉类产品中大肠杆菌的发生率分别为 45%和 52.5%。在大肠杆菌分离株中，肉样中检测到两株 ETEC、一株典型 EPEC 和三株非典型 EPEC，而乳样中仅检测到一株典型 EPEC 和一株非典型 EPEC。
解释和结论
我们的结果表明 ASF 中存在 ETEC 和 EPEC 菌株。未经巴氏消毒的牛奶和在不卫生条件下生产的传统乳制品很可能是 EPEC 血清型和其他人畜共患病原体的主要来源，因此可能对社区健康构成潜在威胁。
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