Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, Bangladesh.
Foodborne Pathog Dis. 2020 Jul;17(7):434-439. doi: 10.1089/fpd.2019.2741. Epub 2020 Jan 8.
In this study, the prevalence and antimicrobial resistance of enterotoxigenic (ETEC) and enteropathogenic (EPEC) were investigated. Altogether 100 stool samples were collected from diarrheal patients attending the Sheikh Hasina Medical College and Hospital, Tangail, Bangladesh, during the period from March 1 to May 30, 2018. pathogenic potential of ETEC and EPEC using a infection model was investigated. Among 100 diarrheal patients, 31% were positive for both ETEC and EPEC strains, 23% were positive for ETEC strains, and 8% were positive for EPEC strains. It was detected that 82.60%, 65.21%, 73.91%, 78.26%, 47.82%, 60.86%, and 47.82% of ETEC strains were resistant to amoxicillin-clavulanic acid (AMC), tetracycline (TE), nalidixic acid (NA), azithromycin, ciprofloxacin, ampicillin (AMP), and erythromycin (E), respectively. Whereas it was detected that 87.5% strains were resistant to AMC, AMP, and E, 75% were resistant to TE and NA, respectively. Both strains developed multidrug resistance to commonly prescribed antibiotics. EPEC showed higher pathogenicity than ETEC as 67.75% and 60% of died after 18 h postinfection with EPEC and ETEC, respectively. The high rate of antimicrobial resistance of EPEC and ETEC highlights the necessity for the prudent use of antimicrobials in Bangladesh.
在这项研究中，调查了肠毒素性（ETEC）和肠致病性（EPEC）的流行情况和抗生素耐药性。2018 年 3 月 1 日至 5 月 30 日期间，从孟加拉国 Tangail 的 Sheikh Hasina 医学院和医院就诊的腹泻患者中采集了 100 份粪便样本。使用感染模型调查了 ETEC 和 EPEC 的致病性。在 100 名腹泻患者中，31%同时携带 ETEC 和 EPEC 菌株，23%携带 ETEC 菌株，8%携带 EPEC 菌株。检测到 82.60%、65.21%、73.91%、78.26%、47.82%、60.86%和 47.82%的 ETEC 菌株分别对阿莫西林-克拉维酸（AMC）、四环素（TE）、萘啶酸（NA）、阿奇霉素、环丙沙星、氨苄西林（AMP）和红霉素（E）耐药。而 87.5%的菌株对 AMC、AMP 和 E 耐药，75%的菌株对 TE 和 NA 耐药。两种菌株对常用抗生素均表现出多重耐药性。EPEC 的致病性高于 ETEC，分别有 67.75%和 60%的 EPEC 和 ETEC 感染的 18 小时后死亡。EPEC 和 ETEC 的高抗生素耐药率突出表明在孟加拉国需要谨慎使用抗生素。
