Madani Arghavan, Esfandiari Zahra, Shoaei Parisa, Ataei Behrooz
Department of Food Science and Technology, Food Security Research Center, School of Nutrition and Food Science, P. O. Box: 81746-73461, Isfahan University of Medical Sciences, Isfahan, Iran.
Nosocomial Infection Research Center, P. O. Box: 81746-73461, Isfahan University of Medical Sciences, Isfahan, Iran.
Foods. 2022 Mar 26;11(7):960. doi: 10.3390/foods11070960.
Diarrheagenic (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of 200 samples, including traditional and pasteurized dairy products, were analyzed. In 200 samples, 54 isolates, including (48/110) and (6/90) positive samples of traditional and pasteurized dairy products, were detected. Furthermore, pathogenic strains were isolated from 30% of traditional dairy products and 5.55% of pasteurized dairy products. Most isolates were classified as enteropathogenic (EPEC). Moreover, antibiotic resistance was evaluated using the disk diffusion method for pathogenic . Overall, 73.68% of contaminated samples by pathogenic strains were resistant to at least one antibiotic. The highest resistance was observed against streptomycin (57.9%), followed by tetracycline (50%). Additionally, all isolates were sensitive to amikacin. For evaluating biofilm formation, the violet crystal assay was applied on a polystyrene microplate well for pathogenic isolates. In total, 68.42% of isolates were able to form biofilms. The presence of in dairy products indicates potential health risks for Iranian consumers. Serious measures are needed to control and prevent the spread of this pathogen.
致泻性大肠杆菌（DEC）菌株是全球胃肠道疾病的重要病因，尤其是在发展中国家。本研究旨在调查伊朗伊斯法罕市乳制品中该菌株的存在情况、抗生素耐药性及潜在的生物膜形成情况。共分析了200份样品，包括传统乳制品和巴氏杀菌乳制品。在200份样品中，检测到54株分离株，其中传统乳制品阳性样品48份（共110份），巴氏杀菌乳制品阳性样品6份（共90份）。此外，从30%的传统乳制品和5.55%的巴氏杀菌乳制品中分离出致病菌株。大多数分离株被归类为肠致病性大肠杆菌（EPEC）。此外，采用纸片扩散法对致病菌株进行抗生素耐药性评估。总体而言，73.68%的致病菌株污染样品对至少一种抗生素耐药。观察到对链霉素的耐药性最高（57.9%），其次是四环素（50%）。此外，所有分离株对阿米卡星敏感。为评估生物膜形成情况，对致病分离株在聚苯乙烯微孔板孔中采用结晶紫试验。总共68.42%的分离株能够形成生物膜。乳制品中该菌株的存在表明对伊朗消费者存在潜在健康风险。需要采取严格措施来控制和预防这种病原体的传播。
