Department of Microbiology and Immunology, Faculty of Medical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Microbiology, Zanjan Islamic Azad University, Zanjan, Iran.
J Med Microbiol. 2014 Mar;63(Pt 3):427-432. doi: 10.1099/jmm.0.064600-0. Epub 2013 Nov 26.
Diarrhoea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Diarrhoeagenic Escherichia coli (DEC) is an emerging agent among pathogens that cause diarrhoea. Between March 2011 and January 2012, a total of 600 stool specimens from children younger than 5 years of age (450 with and 150 without diarrhoea) were investigated for enteroaggregative E. coli (EAEC), enterohaemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC) and enterotoxigenic E. coli (ETEC) using PCR. Antimicrobial susceptibility testing was performed following Clinical and Laboratory Standards Institute guidelines. The prevalence of DEC pathotypes was 30.4% (137 patients) and 12% (18 patients) in the diarrhoea group and the control group, respectively. The most frequently isolated pathotype in diarrhoeal children was ETEC. This pathotype was detected significantly more often in children with diarrhoea (14.4%) than in children without diarrhoea (5.3%). EAEC and EPEC were detected with slightly higher frequencies in children with (8 and 4.2%, respectively) than in children without (4.6 and 2%, respectively) (P>0.05) diarrhoea. EHEC was only detected in children with diarrhoea (3.8%). Of the children from the diarrhoea group, 10% were colonized with more than one DEC pathotype. The DEC isolates exhibited high-level resistance to erythromycin (100%), azteronam (80.7%), amoxicillin (74.4%) and tetracycline (69.3%), and 86.4% of isolates were multidrug resistant. In conclusion, ETEC continues to be an important agent associated with diarrhoea in children from Tabriz, Iran.
腹泻仍然是发展中国家婴幼儿发病率和死亡率的最常见原因之一。产肠聚集性大肠埃希菌（DEC）是引起腹泻的病原体之一。2011 年 3 月至 2012 年 1 月，对 600 份来自 5 岁以下儿童的粪便标本进行了检测，其中 450 份有腹泻，150 份无腹泻，以了解肠聚集性大肠埃希菌（EAEC）、肠出血性大肠埃希菌（EHEC）、肠致病性大肠埃希菌（EPEC）和肠产毒性大肠埃希菌（ETEC）。根据临床和实验室标准协会的指南进行了抗菌药物敏感性测试。腹泻组和对照组 DEC 病原体的流行率分别为 30.4%（137 例）和 12%（18 例）。腹泻儿童中最常分离到的病原体是 ETEC。该病原体在腹泻儿童（14.4%）中检测到的频率明显高于无腹泻儿童（5.3%）。在腹泻儿童（8%和 4.2%）中检测到 EAEC 和 EPEC 的频率略高于无腹泻儿童（4.6%和 2%）（P>0.05）。仅在腹泻儿童中检测到 EHEC（3.8%）。腹泻组的儿童中有 10%定植了一种以上的 DEC 病原体。DEC 分离株对红霉素（100%）、氨曲南（80.7%）、阿莫西林（74.4%）和四环素（69.3%）表现出高水平耐药性，86.4%的分离株为多药耐药。总之，ETEC 仍然是伊朗大不里士儿童腹泻的重要病原体。
