Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People's Republic of China.
Int J Nanomedicine. 2019 Sep 9;14:7353-7362. doi: 10.2147/IJN.S217974. eCollection 2019.
Profiting from the development of nanomaterials, photothermal therapy (PTT) has been discovered as efficient tumor ablation strategy for breast cancer.
Novel oxygen vacancy-rich tungsten bronze nanoparticles (NaWO) were synthesized through a simple pyrogenic decomposition process. TEM, XRD, UV-vis-NIR, photothermal conversion ability, and photothermal stability were performed. The viabilities of 293T and 4T1 cells after treating with 200 μg/mL NaWO nanoparticles for 24 or 48 hrs were both above 80%, which proved the good biosafety and cytotoxicity of NaWO in vitro. Two in vivo breast cancer models, namely percutaneous and intratibial 4T1 models were established and NaWO (20 mg/kg) with power intensity of 1.5 W/cm 980 nm laser photothermal treatment was used in vivo.
We successfully synthesized ~150 nm NaWO nanoparticles with desirable PTT effects, as evidenced by the temperature increase from 25.8°C to 41.8°C in 5 mins under the irradiation of 980 nm laser (1 mg/mL). Also, cellular compatibility of NaWO nanoparticles was found upon physiologic 293T cells, in contrast with significant cytotoxicity against breast cancer 4T1 cell in vitro dose-dependently. Besides, two in vivo breast cancer models showed the decent tumor ablation ability of NaWO nanoparticles, demonstrating percutaneous 4T1 tumor elimination without recurrence during 2 weeks observation as well as intratibial breast cancer inhibition with decreased bone destruction and tumor volume after NaWO+PTT in vivo.
For the first time, we developed a novel oxygen vacancy-rich tungsten bronze nanoparticles (NaWO) through a simple pyrogenic decomposition process for PTT. Both in vitro and in vivo experiments showed the good PTT ability and tumor ablation effects of synthesized NaWO nanoparticles against breast cancer osteolytic bone metastasis. Additionally, our oxygen-deficient NaWO nanoparticles will expand the research horizons of PTT nanomaterials.
得益于纳米材料的发展，光热疗法（PTT）已被发现是一种有效的乳腺癌肿瘤消融策略。
通过简单的热分解过程合成了新型富氧空位的钨青铜纳米粒子（NaWO）。进行了 TEM、XRD、UV-vis-NIR、光热转换能力和光热稳定性测试。293T 和 4T1 细胞在 200μg/mL NaWO 纳米粒子处理 24 或 48 小时后的存活率均高于 80%，这证明了 NaWO 的良好体外生物安全性和细胞毒性。建立了两种体内乳腺癌模型，即经皮和胫骨内 4T1 模型，并在体内使用 20mg/kg NaWO 和 1.5W/cm 980nm 激光光热治疗。
我们成功合成了~150nm 的 NaWO 纳米粒子，具有理想的 PTT 效果，在 980nm 激光（1mg/mL）照射下，5 分钟内温度从 25.8°C 升高到 41.8°C。此外，NaWO 纳米粒子对生理 293T 细胞具有细胞相容性，而在体外则表现出对乳腺癌 4T1 细胞的显著剂量依赖性细胞毒性。此外，两种体内乳腺癌模型显示了 NaWO 纳米粒子良好的肿瘤消融能力，证明了经皮 4T1 肿瘤消除在 2 周观察期间无复发，以及胫骨内乳腺癌抑制，在体内 NaWO+PTT 后骨破坏和肿瘤体积减少。
我们首次通过简单的热分解过程开发了一种新型富氧空位的钨青铜纳米粒子（NaWO）用于 PTT。体内外实验均表明，合成的 NaWO 纳米粒子具有良好的 PTT 能力和肿瘤消融效果，可用于治疗乳腺癌溶骨性骨转移。此外，我们的贫氧 NaWO 纳米粒子将拓宽 PTT 纳米材料的研究视野。
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