慢性肾病患者外周血细胞上补体调节蛋白（CD35、CD55和CD59）的表达水平。
Expression levels of complement regulatory proteins (CD35, CD55 and CD59) on peripheral blood cells of patients with chronic kidney disease.
作者信息
Eldewi Dalia Mahmoud, Alhabibi Alshaymaa M, El Sayed Hanaa Mohammed Eid, Mahmoud Sammar Ahmed Kasim, El Sadek Sanaa Mohammed, Gouda Rasha Mahmoud, Hassan Mohammed Abd El Malik, Ibrahim Amal H, Abd El Haliem Naglaa F
机构信息
Clinical Pathology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Internal Medicine Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
出版信息
Int J Gen Med. 2019 Sep 16;12:343-351. doi: 10.2147/IJGM.S216989. eCollection 2019.
BACKGROUND
Altered regulation of the complement system is associated with multiple kidney diseases. CD35, CD55 and CD59 regulate the complement system, and changes in their expression have previously been linked with kidney disease. This study assessed whether changes in the expression levels of these proteins are associated specifically with chronic kidney disease (CKD) to understand its pathogenesis.
MATERIALS AND METHODS
Sixty CKD patients and 60 age-matched controls were enrolled and divided into two groups: Group I (n=30 pediatric patients and n=30 controls) and Group II (n=30 adult patients and n=30 controls). The expression of CD35, CD55 and CD59 on peripheral blood cells was evaluated by flow cytometry as the proportion of positive cells expressing the marker and mean fluorescence intensity (MFI), also the relation of these markers to the stage of CKD was also evaluated.
RESULTS
Pediatric and adult CKD patients had significantly lower proportion of erythrocytes expressing CD35, CD55 and CD59 than healthy controls (<0.001). In pediatric CKD patients, there was no significant difference in the three studied markers on neutrophils, lymphocytes and monocytes. The changes in expression of CD35, CD55 and CD59 on leukocytes were more pronounced in adult patients, who had lower proportion of CD59-positive neutrophils, CD35- and CD59-positive lymphocytes, and CD59-positive monocytes, as well as lower expression of CD59 on neutrophils and monocytes than adult controls (<0.001, =0.019, <0.001, =0.026, <0.001 and =0.003, respectively). The eGFR directly correlated with the proportion of positivity of some of those markers on peripheral leukocytes while there was inverse correlation between the disease stage and the same markers.
CONCLUSION
There are alterations in the patterns of expression of complement regulatory proteins CD35, CD55 and CD59 on peripheral blood cells of patients with CKD compared with healthy controls.
背景
补体系统调节异常与多种肾脏疾病相关。CD35、CD55和CD59调节补体系统，其表达变化此前已与肾脏疾病相关联。本研究评估这些蛋白表达水平的变化是否与慢性肾脏病（CKD）特异性相关，以了解其发病机制。
材料与方法
纳入60例CKD患者和60例年龄匹配的对照，分为两组：第一组（n = 30例儿科患者和n = 30例对照）和第二组（n = 30例成年患者和n = 30例对照）。通过流式细胞术评估外周血细胞上CD35、CD55和CD59的表达，以表达标志物的阳性细胞比例和平均荧光强度（MFI）表示，同时评估这些标志物与CKD分期的关系。
结果
儿科和成年CKD患者表达CD35、CD55和CD59的红细胞比例显著低于健康对照（<0.001）。在儿科CKD患者中，中性粒细胞、淋巴细胞和单核细胞上这三种研究标志物无显著差异。成年患者白细胞上CD35、CD55和CD59表达的变化更明显，其CD59阳性中性粒细胞、CD35和CD59阳性淋巴细胞以及CD59阳性单核细胞的比例较低，且中性粒细胞和单核细胞上CD59的表达低于成年对照（分别为<0.001、=0.019、<0.001、=0.026、<0.001和=0.003）。估算肾小球滤过率（eGFR）与外周白细胞上部分这些标志物的阳性比例直接相关，而疾病分期与相同标志物呈负相关。
结论
与健康对照相比，CKD患者外周血细胞上补体调节蛋白CD35、CD55和CD59的表达模式存在改变。
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