van Walraven H S, Krab K, Hagendoorn M J, Kraayenhof R
FEBS Lett. 1985 May 6;184(1):96-9. doi: 10.1016/0014-5793(85)80661-x.
Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.
从蓝藻聚球藻6716中提取的脂质中存在的类胡萝卜素，表明了由这些脂质重构的蛋白脂质体以及从同一生物体中分离出的ATP酶复合物中的跨膜电位。缬氨霉素诱导的钾离子扩散电位可使类胡萝卜素吸收带向更长波长移动，而与钾离子梯度的极性无关。与此相反，（外部添加的）探针氧杂萘蓝VI仅在外部钾离子浓度高于内部钾离子浓度时才显示吸收带移动。在没有ATP酶复合物的脂质体中，未观察到类胡萝卜素吸收带移动。
