Lahat N, Hornstein L, Moscona A R, Kalderon N, Pollack S
Immunol Lett. 1985;9(1):43-7. doi: 10.1016/0165-2478(85)90093-8.
Reduced in vitro T cell mitogen-induced transformation, low proportion of T cells and increased proportion of non-T cells were found in blood mononuclear cells of patients with severe burns 3-12 days after the injury. High spontaneous proliferation of non-T cells was observed and could be related mainly to the B cell fraction. Monocytes mediated suppression of mitogen-stimulated T cell proliferation. We further studied the role of monocytes in the enhanced suppressor activity of Con A-activated T cells and found that in this assay system, the patient's T cells mediated suppression in collaboration with monocytes. In vitro, increased suppressor function was probably the result of in vivo stimulation of inhibitory activity ascribed to both monocytes and T cells of patients. Addition of indomethacin to cell cultures markedly reduced suppression of lymphocyte proliferation. Less significant reduction was noted when the patient's T cells were activated in vitro by Con A. Adjuvant treatment of burn patients with indomethacin may play a role in alleviating suppression of immune response in these patients.
在严重烧伤患者受伤后3 - 12天的血液单核细胞中，发现体外T细胞有丝分裂原诱导的转化减少、T细胞比例降低以及非T细胞比例增加。观察到非T细胞有较高的自发增殖，这可能主要与B细胞部分有关。单核细胞介导对有丝分裂原刺激的T细胞增殖的抑制作用。我们进一步研究了单核细胞在伴刀豆球蛋白A（Con A）激活的T细胞增强抑制活性中的作用，发现在该检测系统中，患者的T细胞与单核细胞协同介导抑制作用。在体外，抑制功能增强可能是患者体内单核细胞和T细胞的抑制活性受到刺激的结果。向细胞培养物中添加消炎痛可显著降低淋巴细胞增殖的抑制作用。当患者的T细胞在体外被Con A激活时，抑制作用的降低不太明显。用消炎痛对烧伤患者进行辅助治疗可能在减轻这些患者免疫反应的抑制方面发挥作用。
