Geary T G, Divo A A, Jensen J B
J Protozool. 1985 Feb;32(1):65-9. doi: 10.1111/j.1550-7408.1985.tb03014.x.
A semi-defined minimal medium, in which pantothenic acid is the only vitamin, was used to culture Plasmodium falciparum for the analysis of antimetabolite drugs. Analogs of riboflavin, nicotinamide, pyridoxine, and thiamin inhibited the growth of this parasite; for each drug, effects were much more pronounced after 96 h of exposure compared to 48 h. The most potent drug examined was 8-methylamino-8-desmethyl riboflavin (IC50 value approximately 1.0 X 10(-10) M at 96 h). Avidin, a protein which complexes and thus inactivates biotin, did not affect parasite viability. Other antimalarial drugs, including chloroquine and quinine derivatives and antibiotics, were equipotent in the minimal medium and in RPMI 1640. Four strains of P. falciparum showed only minor differences in sensitivity to these antimetabolites. The use of prolonged drug exposure times and a vitamin-depleted medium allowed the preliminary characterization of antimalarial antimetabolites in vitro.
一种半限定的基础培养基被用于培养恶性疟原虫以分析抗代谢药物，该培养基中泛酸是唯一的维生素。核黄素、烟酰胺、吡哆醇和硫胺素的类似物抑制了这种寄生虫的生长；对于每种药物，与48小时相比，暴露96小时后的效果更为明显。所检测的最有效药物是8-甲基氨基-8-去甲基核黄素（96小时时IC50值约为1.0×10⁻¹⁰ M）。抗生物素蛋白是一种能与生物素结合并使其失活的蛋白质，它不影响寄生虫的活力。其他抗疟药物，包括氯喹和奎宁衍生物以及抗生素，在基础培养基和RPMI 1640中效力相当。四株恶性疟原虫对这些抗代谢物的敏感性仅显示出微小差异。延长药物暴露时间和使用缺乏维生素的培养基使得能够在体外对抗疟抗代谢物进行初步表征。
