Orago A S, Facer C A
Department of Haematology, London Hospital Medical College, UK.
Clin Exp Immunol. 1993 Feb;91(2):287-94. doi: 10.1111/j.1365-2249.1993.tb05897.x.
The addition of recombinant cytokines to Plasmodium falciparum in vitro cultures retarded the growth of the parasite with the effect of recombinant IL-2 (rIL-2) > interferon-gamma (IFN-gamma) > tumour necrosis factor-beta (TNF-beta). The process was concentration dependent, being greatest at 30,000 U/ml and required a 72-h period of continuous exposure for maximum effect. Growth inhibition, as determined morphologically and radiometrically, was a consequence of defective schizont maturation rather than inhibition of merozoite invasion. It was cumulative and detectable within one erythrocytic (48 h) growth cycle.
在体外培养的恶性疟原虫中添加重组细胞因子会抑制疟原虫的生长，重组白细胞介素-2（rIL-2）的作用大于干扰素-γ（IFN-γ），而干扰素-γ又大于肿瘤坏死因子-β（TNF-β）。该过程呈浓度依赖性，在30,000 U/ml时作用最强，且需要连续暴露72小时才能达到最大效果。通过形态学和放射性测定确定的生长抑制是裂殖体成熟缺陷的结果，而非对裂殖子入侵的抑制。这种抑制是累积性的，并且在一个红细胞（48小时）生长周期内即可检测到。
