Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China,
Oncol Res Treat. 2019;42(11):564-571. doi: 10.1159/000502122. Epub 2019 Oct 2.
Cervical cancer is one of the most common malignant tumors among women in the world. MicroRNA-449a (miR-449a) has been identified as a tumor suppressor gene in various cancers. Our present study aimed to explore the biological role of miR-449a in cervical cancer.
The expression of miR-449a in cervical cancer tissues and cell lines was detected using real-time quantitative PCR. Kaplan-Meier survival analysis and a Cox regression assay were used to identify the prognostic significance of miR-449a in cervical cancer. A CCK-8 assay and a Transwell assay were performed to determine the impact of miR-449a on the proliferation, migration, and invasion of cervical cancer cells.
The expression of miR-449a was decreased in both cervical cancer tissues and cell lines. The decreased expression of miR-449a was significantly associated with lymph node metastasis, differentiation, and FIGO stage, as well as with poor survival. In vitro, transfection of miR-449a mimic into cervical cancer cells inhibited cell proliferation, migration, and invasion.
All of the results revealed that miR-449a functions as a suppressor gene and inhibits cell proliferation, migration, and invasion in cervical cancer. In addition, it may serve as a prognostic biomarker for cervical cancer.
宫颈癌是全球女性中最常见的恶性肿瘤之一。microRNA-449a（miR-449a）已被确定为多种癌症中的肿瘤抑制基因。本研究旨在探讨 miR-449a 在宫颈癌中的生物学作用。
采用实时定量 PCR 检测宫颈癌组织和细胞系中 miR-449a 的表达。Kaplan-Meier 生存分析和 Cox 回归分析用于鉴定 miR-449a 在宫颈癌中的预后意义。CCK-8 检测和 Transwell 检测用于确定 miR-449a 对宫颈癌细胞增殖、迁移和侵袭的影响。
miR-449a 在宫颈癌组织和细胞系中的表达均降低。miR-449a 的低表达与淋巴结转移、分化和 FIGO 分期以及不良预后显著相关。体外实验中，miR-449a 模拟物转染宫颈癌细胞后抑制细胞增殖、迁移和侵袭。
所有结果表明，miR-449a 作为一种抑癌基因，抑制宫颈癌中的细胞增殖、迁移和侵袭。此外，它可能作为宫颈癌的预后生物标志物。
