Hamatake R K, Aoyama A, Hayashi M
J Virol. 1985 May;54(2):345-50. doi: 10.1128/JVI.54.2.345-350.1985.
The J protein of phi X174 is a small, highly basic protein and is a component of the phage capsid. We have investigated the role of J protein during single-stranded viral DNA synthesis and phage morphogenesis by using an in vitro system composed of purified viral and host components (Aoyama et al., Proc. Natl. Acad. Sci. U.S.A. 80:4195-4199, 1983). The characterization of the products made in the presence and absence of J protein shows that J protein is not required for viral DNA synthesis, but is required for the packaging of DNA into infectious phage. The ability of J protein to bind to double-stranded DNA as well as single-stranded DNA and other interactions with DNA suggest a model in which J protein binds to double-stranded, replicative form DNA and enters the phage prohead by remaining bound to viral DNA as it is encapsidated.
噬菌体φX174的J蛋白是一种小的、高度碱性的蛋白质，是噬菌体衣壳的一个组成部分。我们通过使用由纯化的病毒和宿主成分组成的体外系统，研究了J蛋白在单链病毒DNA合成和噬菌体形态发生过程中的作用（青山等人，《美国国家科学院院刊》80:4195 - 4199，1983）。对在有和没有J蛋白存在的情况下所产生产物的特性分析表明，病毒DNA合成不需要J蛋白，但将DNA包装成有感染性的噬菌体需要J蛋白。J蛋白与双链DNA以及单链DNA结合的能力以及与DNA的其他相互作用提示了一种模型，即J蛋白与双链复制型DNA结合，并在病毒DNA被包装时保持与它的结合状态进入噬菌体原头部。
