噬菌体φX174的J基因：J蛋白功能的体外分析
The J gene of bacteriophage phi X174: in vitro analysis of J protein function.
作者信息
Hamatake R K, Aoyama A, Hayashi M
出版信息
J Virol. 1985 May;54(2):345-50. doi: 10.1128/JVI.54.2.345-350.1985.
Abstract
The J protein of phi X174 is a small, highly basic protein and is a component of the phage capsid. We have investigated the role of J protein during single-stranded viral DNA synthesis and phage morphogenesis by using an in vitro system composed of purified viral and host components (Aoyama et al., Proc. Natl. Acad. Sci. U.S.A. 80:4195-4199, 1983). The characterization of the products made in the presence and absence of J protein shows that J protein is not required for viral DNA synthesis, but is required for the packaging of DNA into infectious phage. The ability of J protein to bind to double-stranded DNA as well as single-stranded DNA and other interactions with DNA suggest a model in which J protein binds to double-stranded, replicative form DNA and enters the phage prohead by remaining bound to viral DNA as it is encapsidated.
摘要
噬菌体φX174的J蛋白是一种小的、高度碱性的蛋白质，是噬菌体衣壳的一个组成部分。我们通过使用由纯化的病毒和宿主成分组成的体外系统，研究了J蛋白在单链病毒DNA合成和噬菌体形态发生过程中的作用（青山等人，《美国国家科学院院刊》80:4195 - 4199，1983）。对在有和没有J蛋白存在的情况下所产生产物的特性分析表明，病毒DNA合成不需要J蛋白，但将DNA包装成有感染性的噬菌体需要J蛋白。J蛋白与双链DNA以及单链DNA结合的能力以及与DNA的其他相互作用提示了一种模型，即J蛋白与双链复制型DNA结合，并在病毒DNA被包装时保持与它的结合状态进入噬菌体原头部。
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本文引用的文献
1
Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components.噬菌体φX174的形态发生：从纯化的病毒成分体外合成感染性噬菌体。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7285-9. doi: 10.1073/pnas.78.12.7285.
2
DNA packaging by the double-stranded DNA bacteriophages.双链DNA噬菌体的DNA包装
Cell. 1980 Sep;21(2):319-31. doi: 10.1016/0092-8674(80)90468-7.
3
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.一对用于选择双酶切限制片段任一条DNA链的新型M13载体。
Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
4
5
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6
Isolation and some properties of bacteriophage alpha3 gene J mutant.噬菌体α3基因J突变体的分离及某些特性
Mol Gen Genet. 1984;195(3):541-3. doi: 10.1007/BF00341460.
7
Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis.影响脱氧核糖核酸合成的Φ-X-174噬菌体结构突变体。
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8
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9
Studies on the proteins of phi X174. II. The protein composition of the phi X coat.
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10
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