影响脱氧核糖核酸合成的Φ-X-174噬菌体结构突变体。
Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis.
作者信息
Siegel J E, Hayashi M
出版信息
J Virol. 1969 Oct;4(4):400-7. doi: 10.1128/JVI.4.4.400-407.1969.
Abstract
Seven cistrons in phiX-174 were identified and one in particular was studied intensively: cistron A, which is assigned a protein in the mature phage. Amber mutants in this cistron synthesize a new deoxyribonucleic acid (DNA) form in addition to circular phage DNA upon infection of the restrictive host. This DNA is linear, non-infectious, and single-stranded; it is formed from the phage strand of replicative form phiX-174 DNA. These mutants produce two different defective particles in the restrictive host. One particle contains circular phage DNA but is not infectious; the other contains the new DNA form and is similar to the 70S particles found in wild-type phage lysates. The mutant A gene product acts independently of normal A protein upon mixed infection of the restrictive host with an A mutant and a mutant from any other cistron or wild type.
摘要
在φX - 174中鉴定出了7个顺反子，其中一个被特别深入研究：顺反子A，它在成熟噬菌体中编码一种蛋白质。该顺反子中的琥珀突变体在感染限制宿主时，除了环状噬菌体DNA外，还会合成一种新的脱氧核糖核酸（DNA）形式。这种DNA是线性的、无感染性的且为单链；它由复制型φX - 174 DNA的噬菌体链形成。这些突变体在限制宿主中产生两种不同的缺陷颗粒。一种颗粒含有环状噬菌体DNA但无感染性；另一种含有新的DNA形式，类似于在野生型噬菌体裂解物中发现的70S颗粒。当限制宿主与A突变体以及来自任何其他顺反子的突变体或野生型进行混合感染时，突变的A基因产物独立于正常A蛋白发挥作用。
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