Srivastava K C
Prostaglandins Leukot Med. 1985 Mar;17(3):319-27. doi: 10.1016/0262-1746(85)90121-0.
Effects of various concentrations (12.5-500 uM) of linoleic acid and docosahexaenoic acid (C22:6 omega 3)(sod. salt) were examined on the platelet metabolism of labelled arachidonate (AA) under two different incubation conditions. In the first platelets were pretreated with either fatty acid prior to incubation with labelled AA; and in the second incubation platelets were incubated with a mixture containing a fatty acid (linoleic acid or DHA) and labelled AA. At all concentrations the two fatty acids reduced the formation of TxB2. At lower concentrations (up to 200 uM) the fatty acids inhibited platelet cyclooxygenase as shown by a reduced formation of prostaglandins (PGs) and TxB2. At higher concentrations (400 and 500 uM), however, the fatty acids behaved differently. Although TxB2 formation was reduced, there was observed an increased formation of PGs. In DHA pretreated platelets only PGE2 increased (to double control values). Platelets pretreated with linoleic acid produced increased amounts of all PGs (PGF2 alpha, PGE2, PGD2), and this effect was greatest for PGE2 which increased by 5-6 fold of control values. DHA showed a dose-dependent inhibition of platelet aggregation induced by arachidonate, epinephrine and collagen.
在两种不同的孵育条件下，研究了不同浓度（12.5 - 500 μM）的亚油酸和二十二碳六烯酸（C22:6 ω-3，钠盐）对标记花生四烯酸（AA）血小板代谢的影响。第一种情况是在与标记AA孵育之前，先用脂肪酸预处理血小板；第二种情况是将血小板与含有脂肪酸（亚油酸或DHA）和标记AA的混合物一起孵育。在所有浓度下，这两种脂肪酸均降低了血栓素B2（TxB2）的形成。在较低浓度（高达200 μM）时，脂肪酸抑制血小板环氧化酶，这表现为前列腺素（PGs）和TxB2的形成减少。然而，在较高浓度（400和500 μM）时，脂肪酸的表现有所不同。尽管TxB2的形成减少，但观察到PGs的形成增加。在DHA预处理的血小板中，仅前列腺素E2（PGE2）增加（达到对照值的两倍）。用亚油酸预处理的血小板产生的所有PGs（前列腺素F2α、PGE2、前列腺素D2）量均增加，其中PGE2的增加最为显著，增加至对照值的5 - 6倍。DHA对花生四烯酸、肾上腺素和胶原诱导的血小板聚集具有剂量依赖性抑制作用。
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