Li Zhiyuan, Tian Changxu, Huang Yang, Lin Xinghua, Wang Yaorong, Jiang Dongneng, Zhu Chunhua, Chen Huapu, Li Guangli
Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China.
Animals (Basel). 2019 Oct 1;9(10):756. doi: 10.3390/ani9100756.
has high economic value and is one of the most attractive aquaculture species in China. Despite its economic importance, studies of its genome have barely been performed. In this study, we conducted a first genomic survey of using next-generation sequencing (NGS). In total, 45.063 Gb of high-quality sequence data were obtained. For the 17-mer frequency distribution, the genome size was estimated to be 508.50 Mb. The sequence repeat ratio was calculated to be 21.25%, and the heterozygosity ratio was 0.92%. Reads were assembled into 1,009,363 contigs, with a N50 length of 1362 bp, and then into 814,219 scaffolds, with a N50 length of 2173 bp. The average Guanine and Cytosine (GC) content was 45.04%. Dinucleotide repeats (56.55%) were the dominant form of simple sequence repeats (SSR).
具有很高的经济价值，是中国最具吸引力的水产养殖品种之一。尽管其具有经济重要性，但对其基因组的研究却几乎没有开展。在本研究中，我们使用下一代测序（NGS）对 进行了首次基因组调查。总共获得了45.063 Gb的高质量序列数据。对于17聚体频率分布，估计基因组大小为508.50 Mb。计算得出序列重复率为21.25%，杂合率为0.92%。 reads被组装成1,009,363个重叠群，N50长度为1362 bp，然后组装成814,219个支架，N50长度为2173 bp。鸟嘌呤和胞嘧啶（GC）的平均含量为45.04%。二核苷酸重复（56.55%）是简单序列重复（SSR）的主要形式。
