Zhong Qiong, Huang Juncong, Wei Jiawang, Wu Renrui
Department of Oncology, People's Hospital of Ganzhou, No. 18 Mei Guang Avenue, Ganzhou, 341000 Guangdong People's Republic of China.
Cancer Cell Int. 2019 Oct 1;19:252. doi: 10.1186/s12935-019-0959-y. eCollection 2019.
Circular RNA (circRNA) CDR1as plays an important role in the occurrence and development of human tumors. The purpose of this study is to investigate the molecular mechanism of circRNA CDR1as in the development of nasopharyngeal carcinoma (NPC).
The mRNA expressions of circRNA CDR1as, miR-7-5p, and E2F3 were detected by qRT-PCR. The effects of circRNA CDR1as, miR-7-5p, and E2F3 on NPC cells were investigated using cell counting kit-8 (CCK8) method, colony formation assay, and representative metabolite assay. The molecular mechanism of circRNA CDR1 in NPC was studied by bioinformatics and luciferase reporter assay. In addition, the biological activity of circRNA CDR1as was also investigated in NPC xenograft tumor mice model.
The results showed that the circRNA CDR1as expression was significantly up-regulated in NPC tissues by comparison with non-tumor NPE tissues (< 0.01), suggesting that circRNA CDR1as was associated with poor prognosis in NPC patients. Moreover, circRNA CDR1as could up-regulate E2F3 expression by binding miR-7-5p, and promote the growth and glucose metabolism of NPC cells. Meanwhile, circRNA CDR1as could promote NPC progression through the negative regulation of miR-7-5p in the xenograft tumor model.
CircRNA CDR1as promoted the occurrence and development of NPCs by successively up-regulating the expression of miR-7-5p and E2F3, suggesting CircRNA CDR1as as a potential target for the treatment of NPC patients. The study was approved by the cancer center's institutional research ethics committee on Oct 18, 2008 (2008GZ2847462).
环状RNA（circRNA）CDR1as在人类肿瘤的发生发展中起重要作用。本研究旨在探讨circRNA CDR1as在鼻咽癌（NPC）发生发展中的分子机制。
采用qRT-PCR检测circRNA CDR1as、miR-7-5p和E2F3的mRNA表达。采用细胞计数试剂盒-8（CCK8）法、集落形成试验和代表性代谢物试验研究circRNA CDR1as、miR-7-5p和E2F3对NPC细胞的影响。通过生物信息学和荧光素酶报告试验研究circRNA CDR1在NPC中的分子机制。此外，还在NPC异种移植瘤小鼠模型中研究了circRNA CDR1as的生物学活性。
结果显示，与非肿瘤NPE组织相比，NPC组织中circRNA CDR1as表达显著上调（<0.01），提示circRNA CDR1as与NPC患者预后不良有关。此外，circRNA CDR1as可通过结合miR-7-5p上调E2F3表达，促进NPC细胞的生长和糖代谢。同时，在异种移植瘤模型中，circRNA CDR1as可通过对miR-7-5p的负调控促进NPC进展。
CircRNA CDR1as通过依次上调miR-7-5p和E2F3的表达促进NPC的发生发展，提示CircRNA CDR1as是治疗NPC患者的潜在靶点。该研究于2008年10月18日获得癌症中心机构研究伦理委员会批准（2008GZ2847462）。
