Naseri Zahra, Bahmani Nasrin, Alikhani Mohammad Yosef, Hashemi Seyed Hamid, Roshanaei Ghodratollah
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion, Hamadan, Iran.
Zoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Iran J Pathol. 2019 Summer;14(3):206-211. doi: 10.30699/ijp.2019.91536.1888. Epub 2019 Aug 1.
BACKGROUND & OBJECTIVE: Brucellosis is one of the most prevalent bacterial zoonotic diseases which afflicts both humans and animals. Genetic factors play an important role in susceptibility to brucellosis. One of these factors is interferon-gamma (IFN-), which is vital in the defense mechanism against infectious diseases such as brucellosis. The purpose of this study was to evaluate the relationship between two single nucleotide polymorphisms (SNPs) at positions -611 and -56 within the promoter region of interferon-gamma receptor-1 gene (IFN- R1) and brucellosis.
In this research, the genomic DNA was collected from 60 peripheral blood samples infected with brucellosis and 68 healthy volunteers. DNA was extracted by salting out method. Then, DNA genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP).
The results showed that there is a significant difference in -611 SNP frequencies between control and patient groups. At position -611, CC genotype was related to patient group (=0.024) and TT genotype was related to the control group. According to the results, males had a higher frequency of infection.
The presence of C allele in position -611 in IFNγ R1 gene promoter was related to a higher risk of disease and susceptibility to brucellosis. Moreover, the presence of T allele in position γ.
布鲁氏菌病是最常见的人畜共患细菌性疾病之一，会感染人类和动物。遗传因素在布鲁氏菌病易感性中起重要作用。其中一个因素是干扰素-γ（IFN-γ），它在抵御布鲁氏菌病等传染病的防御机制中至关重要。本研究的目的是评估干扰素-γ受体-1基因（IFN-γR1）启动子区域-611和-56位点的两个单核苷酸多态性（SNP）与布鲁氏菌病之间的关系。
在本研究中，从60份布鲁氏菌病感染患者的外周血样本和68名健康志愿者中收集基因组DNA。采用盐析法提取DNA。然后，使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析DNA基因型。
结果显示，对照组和患者组在-611 SNP频率上存在显著差异。在-611位点，CC基因型与患者组相关（P=−0.024），TT基因型与对照组相关。根据结果，男性感染频率更高。
IFNγR1基因启动子-611位点存在C等位基因与疾病风险增加和布鲁氏菌病易感性有关。此外，γ位点存在T等位基因。
