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干扰素-γ受体-1基因启动子多态性与麦加地区布鲁氏菌病易感性

Interferon-γ receptor-1 gene promoter polymorphisms and susceptibility for brucellosis in Makkah region.

作者信息

Ismael A B, Mergani A, Salim A, Mostafa S, Alkafaween I

机构信息

Department of Medical Biotechnology, Faculty of Applied Medical Sciences, Taif University, Turrabah, 21995, KSA.

Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.

出版信息

Afr Health Sci. 2018 Dec;18(4):1157-1165. doi: 10.4314/ahs.v18i4.36.

DOI:10.4314/ahs.v18i4.36
PMID:30766581
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6354847/
Abstract

BACKGROUND

Genetic polymorphisms that affect the production levels of certain cytokines and/or their receptors may determine the risk, severity or protection in some infectious diseases like brucellosis.

OBJECTIVES

The aim of this study was to investigate the association of certain known Interferon-γ Receptor-1 (IFN-γ R1) gene promoter polymorphisms and the susceptibility to infection with Brucellosis in Saudi population.

METHODS

A cases-control association study was conducted in 69 individuals with human brucellosis and 94 healthy individuals. Genotyping of IFN-γ R1 - 56 C>T and IFN-γ R1 - 611 A>G polymorphism in both patients and healthy controls was done by PCR- restriction enzyme length polymorphisms (PCR-RFLP) and PCR- confronting two primer pairs (PCR-CTPP) methods and were assessed for potential associations with susceptibility for human brucellosis and their mode of penetrance.

RESULTS

Interestingly, we have designed a PCR-CTPP system to be used for genotyping of IFN-γ R1 - 611 A > G polymorphism. The PCR-CTPP is an accurate method for genotyping of SNPs. Moreover, it is time-saving, inexpensive and easy to perform.

CONCLUSION

Both tested polymorphisms, IFN-γ R1 - 56 C>T and IFN-γ R1 -611 A>G polymorphism had no role in genetic susceptibility to human brucellosis in the study population. The PCR-CTPP can be used for genotyping IFN-γ R1 - 611 A > G polymorphism and other types of mutation.

摘要

背景

影响某些细胞因子及其受体产生水平的基因多态性可能决定某些传染病（如布鲁氏菌病）的风险、严重程度或保护性。

目的

本研究旨在调查沙特人群中某些已知的干扰素-γ受体1（IFN-γR1）基因启动子多态性与布鲁氏菌病感染易感性之间的关联。

方法

对69例人类布鲁氏菌病患者和94例健康个体进行病例对照关联研究。通过聚合酶链反应-限制性酶切片段长度多态性（PCR-RFLP）和聚合酶链反应-双引物对法（PCR-CTPP）对患者和健康对照者的IFN-γR1 - 56 C>T和IFN-γR1 - 611 A>G多态性进行基因分型，并评估其与人类布鲁氏菌病易感性及其穿透模式的潜在关联。

结果

有趣的是，我们设计了一种PCR-CTPP系统用于IFN-γR1 - 611 A > G多态性的基因分型。PCR-CTPP是一种准确的单核苷酸多态性基因分型方法。此外，它省时、廉价且易于操作。

结论

在研究人群中，所检测的两种多态性，即IFN-γR1 - 56 C>T和IFN-γR1 -611 A>G多态性在人类布鲁氏菌病的遗传易感性中不起作用。PCR-CTPP可用于IFN-γR1 - 611 A > G多态性及其他类型突变的基因分型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/a51438f103cb/AFHS1804-1157Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/c92c62d8802b/AFHS1804-1157Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/08e99fd25d53/AFHS1804-1157Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/7077ca69f2ab/AFHS1804-1157Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/a51438f103cb/AFHS1804-1157Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/c92c62d8802b/AFHS1804-1157Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/08e99fd25d53/AFHS1804-1157Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/7077ca69f2ab/AFHS1804-1157Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa13/6354847/a51438f103cb/AFHS1804-1157Fig4.jpg

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