Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dig Dis Sci. 2010 Jul;55(7):2008-13. doi: 10.1007/s10620-009-0989-8. Epub 2009 Oct 1.
The purpose of this study was to investigate vitamin D receptor (VDR) gene ApaI and TaqI polymorphisms in relation to the risk of colorectal cancer (CRC) in the Iranian population.
Two single nucleotide polymorphisms in intron 8 (ApaI) and exon 9 (TaqI) of the VDR gene were genotyped in 160 subjects with CRC and 180 controls by use of the PCR-RFLP method.
The VDR ApaI genotype distribution differed significantly between subjects with CRC and controls. The ApaI "aa" genotype compared with "AA" genotype was associated with a 2.3-fold increased risk of CRC (P = 0.014; OR = 2.25, 95% CI = 1.18-4.28) and the difference remained significant after adjustment for age, BMI, sex, and smoking status (P = 0.014; OR = 2.32, 95% CI = 1.19-4.54). Furthermore, the ApaI "a" allele was significantly overrepresented in subjects with CRC than controls (P = 0.014; OR = 1.48, 95% CI = 1.08-2.02).
These findings suggest that VDR ApaI genotype "aa" is associated with increased risk of CRC in the Iranian population. However, these data remain to be confirmed in larger studies and in other populations.
本研究旨在探讨维生素 D 受体（VDR）基因 ApaI 和 TaqI 多态性与伊朗人群结直肠癌（CRC）风险的关系。
采用 PCR-RFLP 法检测 160 例 CRC 患者和 180 例对照者 VDR 基因内含子 8（ApaI）和外显子 9（TaqI）的 2 个单核苷酸多态性。
CRC 患者与对照组 VDR ApaI 基因型分布差异有统计学意义。与“AA”基因型相比，ApaI“aa”基因型 CRC 的风险增加了 2.3 倍（P = 0.014；OR = 2.25，95%CI = 1.18-4.28），且在调整年龄、BMI、性别和吸烟状况后差异仍有统计学意义（P = 0.014；OR = 2.32，95%CI = 1.19-4.54）。此外，CRC 患者中 ApaI“a”等位基因明显高于对照组（P = 0.014；OR = 1.48，95%CI = 1.08-2.02）。
这些发现提示 VDR ApaI 基因型“aa”与伊朗人群 CRC 风险增加有关。然而，这些数据仍需在更大的研究和其他人群中得到证实。
