Pidddubnyi Artem, Romaniuk Anatolii, Radomychelski Inna-Margaryta, Moskalenko Yuliia, A Moskalenko Roman
Department of Pathology, Medical Institute, Sumy State University, Sumy, Ukraine.
Department of Surgery and Oncology, Medical Institute, Sumy State University, Sumy, Ukraine.
Iran J Pathol. 2019 Summer;14(3):212-222. doi: 10.30699/ijp.2019.91400.1884. Epub 2019 Aug 1.
BACKGROUND & OBJECTIVE: To study the immunophenotype of prostate cancer (PC) with the presence and absence of intraluminal inclusions (IIn), depending on the grade score.
A total of 30 PC samples with IIn (group E) and 30 PC samples without them (group C) were studied. These groups were divided into 2 subgroups, depending on the grade of malignancy, which was determined according to the Gleason score as moderate and high-grade tumors. Macroscopic analysis, hematoxylin-eosin staining, immunohistochemistry (androgen receptors, p53 and Bax proteins, Hsp70 and Hsp90, CD68, VEGF, OSN, MMP-1) were used.
The expression level of VEGF was higher in the more differentiated tumors of the control group (<0.01). Increased expression of prognostic-adverse markers p53 (in the presence of IIn, <0.01) and MMP-1 (<0.05) was observed. Also, a higher level of OSN expression was found in PC tissue with IIn (<0.01) due to its participation in the processes of biomineralization. The expression level of CD68 and Bax protein was higher in the PC group with IIn (both <0.01). Furthermore, Hsp90 had a significantly lower expression level in the PC of group E (<0.05).
the presence of IIn in the PC samples of group E promotes tissue remodeling with mechanical trauma, chronic inflammation, and fibrosis development. The presence of IIn in PC leads to the increase of OSN, CD68 and Bax expression and decrease of Hsp90 and VEGF expression. High expression of p53 and MMP-1 and low expression of OSN and VEGF was identified as a characteristic of high-grade tumors.
根据分级评分研究存在和不存在腔内包涵体（IIn）的前列腺癌（PC）的免疫表型。
共研究了30例有IIn的PC样本（E组）和30例无IIn的PC样本（C组）。根据恶性程度将这些组分为2个亚组，恶性程度根据Gleason评分确定为中度和高级别肿瘤。采用宏观分析、苏木精-伊红染色、免疫组织化学（雄激素受体、p53和Bax蛋白、Hsp70和Hsp90、CD68、VEGF、OSN、MMP-1）。
对照组中分化程度较高的肿瘤中VEGF的表达水平更高（<0.01）。观察到预后不良标志物p53（存在IIn时，<0.01）和MMP-1（<0.05）的表达增加。此外，由于其参与生物矿化过程，在有IIn的PC组织中发现OSN表达水平更高（<0.01）。有IIn的PC组中CD68和Bax蛋白的表达水平更高（均<0.01）。此外，E组PC中Hsp90的表达水平显著较低（<0.05）。
E组PC样本中IIn的存在促进了伴有机械性创伤、慢性炎症和纤维化发展的组织重塑。PC中IIn的存在导致OSN、CD68和Bax表达增加，Hsp90和VEGF表达降低。p53和MMP-1的高表达以及OSN和VEGF的低表达被确定为高级别肿瘤的特征。
