Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India.
Bioresour Technol. 2019 Dec;294:122190. doi: 10.1016/j.biortech.2019.122190. Epub 2019 Sep 24.
This study report kinetics of PAHs and crude oil degradation by a newly isolated multiple heavy metal tolerant Novosphingobium panipatense P5:ABC. The isolate showed hydrocarbon degrading enzyme activities namely alkane hydroxylase, catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. The level of C23O activity was 9.63 times higher than C12O thus suggesting active involvement of meta-cleavage pathway. The data of biodegradation of hydrocarbons fitted well to the first order kinetic model. The degradation rate was highest for phenanthrene followed by crude oil, and fluoranthene. We have further reported the estimate of fundamental kinetic parameters, half-saturation constant (K) and maximum degradation rates (V) for biodegradation of phenanthrene and fluoranthene. Overall characterization underscores the potential of Novosphingobium in bioremediation of crude oil polluted sites.
本研究报告了一种新分离的多重金属耐受新鞘氨醇单胞菌 P5:ABC 对 PAHs 和原油降解的动力学。该分离株表现出烃降解酶活性，即烷烃羟化酶、儿茶酚 1,2-双加氧酶和儿茶酚 2,3-双加氧酶。C23O 的活性水平比 C12O 高 9.63 倍，这表明间位裂解途径的活跃参与。烃类生物降解的数据很好地拟合了一级动力学模型。菲的降解速率最高，其次是原油和荧蒽。我们进一步报道了生物降解菲和荧蒽的基本动力学参数、半饱和常数（K）和最大降解速率（V）的估算。总体特征强调了新鞘氨醇单胞菌在原油污染场地生物修复中的潜力。
