College of Wildlife and Protected Area, Northeast Forestry University, PR China; Center of Conservation Medicine and Ecological Safety, Northeast Forestry University, PR China.
Vet Microbiol. 2019 Oct;237:108390. doi: 10.1016/j.vetmic.2019.108390. Epub 2019 Aug 16.
Parvovirosis is a highly contagious disease in dogs, often causing acute hemorrhagic enteritis and altering the intestinal microflora. In this study, real-time PCR was used to detect the viral copy numbers in dogs diagnosed with the disease. Hematological and hemobiochemical parameters were also determined. The species and abundances of the fecal microbial flora in both sick and healthy dogs were determined and compared via metagenomic sequencing. The viral copy numbers in the sick dogs were infected with little difference in the positive samples. The blood coagulation time was significantly shorter and the number of white blood cells was significantly greater in the sick dogs. The serum calcium content was slightly increased and the phosphorus content was reduced in the sick dogs. The LDH and CK activities were significantly elevated in the sick dogs. Metagenomic sequencing and analysis revealed relatively more Escherichia, Lachnoclostridium, gnavus group (Ruminococcus), and uncultured_bacterium_f_lachnospiraceae in the infected dogs, whereas the abundance of Collinsella was relatively reduced. Alloprevotella and Sutterella were absent among the fecal microorganisms of the infected dogs. The relative abundances of Romboutsia, Erysipelatoclostridium, Anaerotruncus, and Blautia were significantly increased in the infected dogs. Functional analysis of the metagenomes of the samples indicated a significant enrichment of the 'replication, recombination and repair', 'nucleotide transport and metabolism', 'transcription', and 'defense metabolism' functions in the fecal microbial flora of the infected dogs. In summary, this study provides a scientific theoretical basis for preventing and controlling diarrhea caused by the canine parvovirus.
细小病毒性肠炎是一种高度传染性疾病，常引起犬急性出血性肠炎，并改变肠道微生物群落。在本研究中，使用实时 PCR 检测了诊断为该疾病的犬的病毒拷贝数。还测定了血液学和血液生化学参数。通过宏基因组测序确定了患病和健康犬粪便微生物群落的种类和丰度，并进行了比较。患病犬的病毒拷贝数感染阳性样本无明显差异。患病犬的凝血时间明显缩短，白细胞数量明显增加。患病犬的血清钙含量略有增加，磷含量降低。患病犬的 LDH 和 CK 活性显著升高。宏基因组测序和分析显示，感染犬体内的大肠杆菌、lachnoclostridium、gnavus 组（ruminococcus）和未培养 bacterium_f_lachnospiraceae 相对较多，而柯林斯氏菌的丰度相对减少。感染犬的粪便微生物中不存在 alloprevotella 和 sutterella。感染犬体内romboutsia、erysipelatoclostridium、anaerotruncus 和 blautia 的相对丰度显著增加。样品的宏基因组功能分析表明，感染犬粪便微生物群落中“复制、重组和修复”、“核苷酸转运和代谢”、“转录”和“防御代谢”功能显著富集。总之，本研究为预防和控制犬细小病毒引起的腹泻提供了科学理论依据。
