Oba Patrícia M, Swanson Olivia R, Kang Yifei, Mioto Julio C, Menton John F, Vinay Elena, Millette Mathieu, Kelly Melissa R, Swanson Kelly S
Department of Animal Sciences, University of Illinois Urbana-Champaign, Urbana, IL, United States.
Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, United States.
Front Vet Sci. 2025 Jul 17;12:1617072. doi: 10.3389/fvets.2025.1617072. eCollection 2025.
Abrupt dietary transitions are common in pets, but can lead to digestive disturbances, altered gut microbiota composition, and impaired intestinal integrity. The consumption of live microorganisms may have potential to mitigate these effects by stabilizing the gut microbiota and enhancing intestinal functionality. The current study aimed to evaluate the effects of ATCC PTA-122264 supplementation on fecal characteristics, microbiota composition, and dysbiosis index of dogs undergoing an abrupt dietary change. Twelve healthy adult spayed female beagle dogs (6.0 ± 1.14 yr; 8.7 ± 0.91 kg body weight) were used in a replicated 3 × 3 Latin square design. In each experimental period, dogs were allotted to one of three treatments and fed a high-fiber kibble diet for 28 d: (1) 250 mg/d of maltodextrin (control), (2) 1 × 10 colony-forming units (CFU)/d of , or (3) 5 × 10 CFU/d of . All dogs were then abruptly transitioned to a high-protein, high-fat canned diet and fed for 14 d. Fresh fecal samples were collected before (d 0) and 2, 6, 10, and 14 d after the diet change for fecal scoring, pH, dry matter (DM) content, and microbiota analysis. Data were statistically analyzed to identify differences due to treatment, time, and treatment*time interactions, with < 0.05 accepted as being significant. Diet change did not impact fecal pH or scores but reduced fecal DM percentage and bacterial alpha diversity measures. Bacterial beta diversity analysis revealed a distinct shift in the microbial community following the diet transition. Diet change reduced ( < 0.05) the abundances of short-chain fatty acid (SCFA)-producing bacteria and increased ( < 0.05) the relative abundance of potentially pathogenic bacteria, resulting in an elevated ( < 0.05) dysbiosis index. supplementation did not attenuate the microbial shifts caused by the diet transition. These findings confirm that an abrupt diet change significantly impacts some stool characteristics and fecal microbiota populations of dogs. Further investigation of spp. strains and dosages is required to determine the potential benefits that they may provide during dietary transition.
宠物饮食突然转变的情况很常见，但可能会导致消化紊乱、肠道微生物群组成改变以及肠道完整性受损。食用活微生物可能有潜力通过稳定肠道微生物群和增强肠道功能来减轻这些影响。本研究旨在评估补充ATCC PTA - 122264对经历突然饮食变化的犬类粪便特征、微生物群组成和失调指数的影响。12只健康成年已绝育雌性比格犬（6.0±1.14岁；体重8.7±0.91千克）用于重复的3×3拉丁方设计。在每个实验阶段，将犬只分配到三种处理之一，并喂食高纤维干狗粮28天：（1）每天250毫克麦芽糊精（对照），（2）每天1×10菌落形成单位（CFU）的[具体菌种未给出]，或（3）每天5×10 CFU的[具体菌种未给出]。然后所有犬只突然转变为高蛋白、高脂肪罐装饮食并喂食14天。在饮食改变前（第0天）以及饮食改变后第2、6、10和14天收集新鲜粪便样本，用于粪便评分、pH值、干物质（DM）含量和微生物群分析。对数据进行统计分析以确定处理、时间和处理*时间交互作用引起的差异，P < 0.05被认为具有显著性。饮食改变未影响粪便pH值或评分，但降低了粪便DM百分比和细菌α多样性指标。细菌β多样性分析显示饮食转变后微生物群落发生了明显变化。饮食改变降低了（P < 0.05）产生短链脂肪酸（SCFA）细菌的丰度，并增加了（P < 0.05）潜在致病菌的相对丰度，导致失调指数升高（P < 0.05）。[具体菌种未给出]的补充并未减轻饮食转变引起的微生物变化。这些发现证实，突然的饮食变化会显著影响犬类的一些粪便特征和粪便微生物群。需要进一步研究[具体菌种未给出]菌株和剂量，以确定它们在饮食转变期间可能提供的潜在益处。
