Kilburn-Kappeler Logan R, Doerksen Tyler, Lu Andrea, Palinski Rachel M, Lu Nanyan, Aldrich Charles G
Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USA.
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
Vet Sci. 2023 Sep 2;10(9):553. doi: 10.3390/vetsci10090553.
Corn-fermented protein (CFP), a co-product from the ethanol industry, is produced using post-fermentation technology to split the protein and yeast from fiber prior to drying. The objective of this study was to determine the effect of CFP compared to traditional ingredients on the fecal microbiota of dogs. The four experimental diets included a control with no yeast and diets containing either 3.5% brewer's dried yeast, 2.5% brewer's dried yeast plus 17.5% distiller's dried grains with solubles, or 17.5% CFP. The experimental diets were fed to adult dogs ( = 12) in a 4 × 4 replicated Latin square design. Fresh fecal samples ( = 48) were analyzed by 16S metagenomic sequencing. Raw sequences were processed through mothur. Community diversity was evaluated in R. Relative abundance data were analyzed within the 50 most abundant operational taxonomic units using a mixed model of SAS. Alpha and beta diversity were similar for all treatments. Predominant phyla among all samples were Firmicutes (73%), Bacteroidetes (15%), Fusobacteria (8%), and Actinobacteria (4%). There were no quantifiable ( > 0.05) shifts in the predominant phyla among the treatments. However, nine genera resulted in differences in relative abundance among the treatments. These data indicate that compared to traditional ingredients, CFP did not alter the overall diversity of the fecal microbiota of healthy adult dogs over 14 days.
玉米发酵蛋白（CFP）是乙醇行业的一种副产品，采用发酵后技术生产，在干燥前将蛋白质和酵母与纤维分离。本研究的目的是确定与传统成分相比，CFP对犬类粪便微生物群的影响。四种实验日粮包括不含酵母的对照日粮以及分别含有3.5%啤酒干酵母、2.5%啤酒干酵母加17.5%含可溶物的干酒糟或17.5%CFP的日粮。实验日粮以4×4重复拉丁方设计喂给成年犬（n = 12）。通过16S宏基因组测序分析新鲜粪便样本（n = 48）。原始序列通过mothur进行处理。在R软件中评估群落多样性。使用SAS的混合模型在50个最丰富的操作分类单元内分析相对丰度数据。所有处理的α多样性和β多样性相似。所有样本中的主要门类为厚壁菌门（73%）、拟杆菌门（15%）、梭杆菌门（8%）和放线菌门（4%）。各处理之间主要门类没有可量化的（P>0.05）变化。然而，有9个属在各处理之间的相对丰度存在差异。这些数据表明，与传统成分相比，在14天内CFP不会改变健康成年犬粪便微生物群的总体多样性。
